Cell sorting via advanced cell sorting instrumentation is performed with high specificity and high standards. Researchers like using the flow cytometry methods because it is the quickest and most effective way to apply measurement to the entire cell process. The operator predetermines or selects certai...
Discover how this flow cytometry system can transform your lab with outstanding performance, automation and standardization. Request a Quote On This Page Discover the BD FACSLyric™ Flow Cytometer difference. Witness results you have never seen before through high sensitivity and improved resolution Str...
interactions they have. Flow cytometry also can be used to measure membrane potential, or the charge difference across the membrane generated by the relative internal and external concentrations of ions such as potassium, sodium and chloride. Changes in membrane potential play a critical role in many...
We then used specific antibodies against intracellular markers such as Tuj-1 or GFAP to discriminate neurons from astrocytes by flow cytometry. The labelling was specific and reliable, allowing quantitative studies. Indeed, we did not find any significant difference in the number of Tuj-1 and GFAP...
Next, we showed that light-chain-mediated capture enables both the interrogation of antigen binding and the detection of immobilized antibodies by flow cytometry (Fig.1c). We captured a commercial anti-streptavidin antibody (IgG) in VHH-functionalized BG-agarose beads and stained the beads with bo...
Unspecific products can be removed, to some extent, by gel purification if there is sufficient size difference between them and the target sequences, or alternatively the handle sequences can be omitted in the PCR and the A, B handle sequences can be added through more laborious linker ...
Refer to: Data file standard for flow cytometry. Data File Standards Committee of the International Society for Analytical Cytology. Cytometry. 1990;11(3):323-332. 30 BD FACSCalibur Instructions for Use BD FACSComp Software Use BD FACSComp software to monitor instrument performance, and to ...
We then used specific antibodies against intracellular markers such as Tuj-1 or GFAP to discriminate neurons from astrocytes by flow cytometry. The labelling was specific and reliable, allowing quantitative studies. Indeed, we did not find any significant difference in the number of Tuj-1 and GFAP...
The difference between PharmLyse™ and Optilyse C (p = 0.93), PharmLyse™ and \\\{IOTest\\\} 3 (p = 0.31), and Optilyse C and \\\{IOTest\\\} 3 (p = 0.34) was not significant. These results emphasize the importance of harmonization of red cell lysis protocols...
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