轻链的C区与V区均由β-sheet方式折叠成两团,对可变区氨基酸进行测序发现,其中某些区域的氨基酸基团可变程度远远高于周围,将这些区域称为超变区(hypervariable region, HV)三维结构上,这些超变区位于β-sheet的连接环(loop)处,由于它们与抗原结合,故被命名为互补决定区域(comp...
重链抗体具有广泛应用价值的是单域重链抗体(single domainantibody,sdAb)部分。单域重链抗体是指仅由重链抗体可变区(Variable region)组成的基因工程抗体,又称为VHH抗体(variable domain of heavy chain of heavy-chain antibody,VHH antibody)或纳米抗体(Nanobody,Nb),分子量为12-15 kD...
轻链的C区与V区均由β-sheet方式折叠成两团,对可变区氨基酸进行测序发现,其中某些区域的氨基酸基团可变程度远远高于周围,将这些区域称为超变区(hypervariable region, HV)三维结构上,这些超变区位于β-sheet的连接环(loop)处,由于它们与抗原结合,故被命名为互补决定区域(complementarity-determining region, CDR)。 ...
Fc-MST-HN, designed for enhanced FcRn binding capacity, has not been evaluated in the context of a full-length antibody, and the structural properties of the attached Fab regions might affect the FcRn-mediated intracellular trafficking pathway. Here we present a comprehensive comparative analysis of...
This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Adalimumab. This product is for research use only. Clonality Monoclonal Host Human Isotype IgG1 Applications for TNF-alpha Antibody (Hu7) [mFluor Violet 610 SE] Application Recommended Usage ...
scFv fragment consists of the variable domain of the heavy and light chains of a monoclonal antibody, usually expressed as one polypeptide chain with a linker in between the chains. Fab fragment is the variable domain of each chain, plus the first constant region. In the case of the Fab for...
The extracellular region of LAG-3 and the CD4 co-receptor share ~20% aa sequence homology but are structurally similar and both bind to major histocompatibility complex class II (MHCII) on antigen-presenting cells (APCs), although LAG-3 has much higher affinity (1, 3). LAG-3 is highly ...
We used a novel combination of small-angle x-ray scattering (SAXS), atomistic molecular dynamic simulations, single-molecule Förster resonance energy transfer, and aggregation-prone region predictions, to characterize structural changes in a native humanized Fab A33 antibody fragment, that correlated ...
Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa. ...
(HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of this receptor was found to bind to several TRAF family members, which may mediate the signal transduction pathways that ...