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Download F1 2009 (Japan) ISO ROM for PSP to play on your pc, mac, android or iOS mobile device.
To determine the membrane topology of LDAF1, we performed fluorescence protease protection assays with LDAF1 fused to EGFP either at the N- or C-terminus (Lorenz et al., 2006). While the luminal ER marker mCherry-ER3 was protected from proteinase K treatment after selective permeabilization of...
We cloned the N-terminus (1–277 aa) of zebrafish foxo3b, corresponding to the 1–304 aa region of mammalian Foxo3a, into the expression vector pCGN-HAM, and vector Psp64 poly(A) (Promega) (for synthesizing mRNA). First of all, we confirmed the dominant negative form activity of the ...
(DMEM + 10% FBS), one day before transfection, HEK293T cells were sub-cultured into 10 cm dish (Corning) at a density of 6 × 106 cells, after 16 h, the cells were transiently co-transfected with packaging vector psPAX2 (Addgene) and envelope vector pMD2.G (Addgene) by...
pLVX-IRES-puro, pLVX-IRES-puro-YTHDF1, pLVX-IRES-puro-Ythdf1, pLVX-IRES-puro-ZNF839, and pLVX-IRES-puro-Zfp839 were transfected into the HEK293T viral packaging cell line together with pSPAX2 and pMD 2.G. Exactly 48 h after transfection, the cells were harvested for RT-PCR or ...
v For information about installation, see these documents: – z/OS Program Directory – Preventative Service Planning (PSP) bucket (available by using IBMLINK) – Softcopy Installation Memo (for Bookmanager publications) – ServerPac: Installing Your Order, if you use the ServerPac method to ...
附件1 评标办法 评标办法(综合评估法) 评标办法前附表 条款号 评审因素 评审标准 本条细化为: 本次评标采用综合评估法.评标委员会对满足招标文件实质 性要求的投标文件,分别按照本章第2.2款规定的评分标准进 行打分,并按综合得分由高到低顺序推荐中标候选人,但投标 报价低于其成本的除外.综合得分相等时,评标委员...
省道S550 马义公路峰峰段改建工程施工 招标公告附件 8.附件 附件 1:资格审查条件 附件 2:评标办法 附件 1,资格审查条件 附录 1 资格审查条件(资质最低要求) 标段号 资质要求 SG-1 具备独立企业法人资格,持有有效的营业执照. 具有住房和城乡建设行政主管部门颁发的公路工程施工总承包二级及以上资 SG-2 质. 具有...