线粒体呼吸链复合物V,通常称为ATP合成酶(ATP synthase)、F型ATP酶(F type ATPase)和F1F0ATP酶(F1F0ATPase),是线粒体氧化磷酸化的终极反应。。其分子量为500KD,含有十六个亚单位,其中两 个:ATP酶6和8为线粒体DNA编码的。ATP酶主要有朗格结构域:F0为质子通道,由几个膜蛋白构成,包括a、b、c、d、e、F6...
When detached from the membrane, the purified F 1 sector functions mainly as an ATPase. In chloroplasts, the synthesis of ATP is also driven by a proton motive force, and the enzyme complex responsible for this synthesis is similar to the mitochondrial and bacterial ATP synthases. The synthesis...
coli and V. alginolyticus was found using antibodies against subunits a , b and c of the E. coli F 0 F 1 -ATPase. The F 0 F 1 -complex from V. alginolyticus was reconstituted into proteoliposomes, which were competent in ATP-dependent proton uptake. This process was inhibited by ...
摘要: PROBLEM TO BE SOLVED: To provide a protein complex having an F0F1-ATPase activity, a DNA encoding the protein complex, a method for producing a protein complex having an F0F1-ATPase activity using the DNA, and a method for producing nucleoside 5'-triphosphate using the protein....
coli [52] and formation of an active α3β3γ cytosolic complex in an E. coli strain over-expressing the respective subunits has been reported recently [169]. Accumulation of such catalytic F1 intermediates has to be prevented during assembly since wasteful ATPase activity leads to a lowered ...
关键词:FoFI.ATPase结构纯化编码11IAbstractATPsynthase,orFoFI—ATPaseisakeyenzymeincellularenergymetabolize.Itisacomplexwithseveralsubunitsandwidelydistributedinalmostallkindsoforganisms.Accordingitspolarityitcanbedividedintotwomajorsectionswhichalehydrophiliesectionoutsidethemembraneandhydrophobicsectiononthemembrane,...
Morikami,A. and Nakamura,K. (1993) Transcript map of oppositely oriented pea mitochondrial genes encoding the α-subunit and the subunit 9 of F1F0-ATPase complex. Biosci. Biotechnol. Biochem., 57, 1530-1535.Morikami, A., Nakamura, K. 1993. Transcript map of oppositely oriented pea ...
1The purified F0 part of the ATP synthase complex from Escherichia coli was incorporated into liposomes and chemically modified by various reagents. The modified F0-liposomes were assayed for H+ uptake and, after reconstitution with F1, for total and dicyclohexylcarbodiimide-sensitive ATPase activity....
Properties of the catalytic (αβ)-core complex of chloroplast CF1-ATPase The CF1(αβ)-core complex previously isolated from the spinach chloroplast CF0F1-ATP synthase contains equal amounts of CF1α- and β-subunits, functions ... Z Gromet-Elhanan,S Avital - Biochimica et Biophysica Acta...
Zheng, J. & Ramirez, V. D. Piceatannol, a stilbene phytochemical, inhibits mitochondrial F0F1-ATPase activity by targeting the F1 complex.Biochem. Biophys. Res. Commun.261 Burnstock, G. & Verkhratsky, A. Long-term (trophic) purinergic signalling: purinoceptors control cell proliferation, diff...