0.1% (w/v) in chloroform) (Sigma, Aldrich, MI) was dissolved in 4 ml of DCM. Subsequently, 5 µg of IL-2 and 150 µl (R&D Systems, Minneapolis MN) of deionized water was added to the organic phase. Next
DeVries DR, Frie RV (1996) Determination of age and growth. In: Murphy BR, Willis DW (eds) Fisheries techniques, 2nd edn. American Fisheries Society, Bethesda, MD, pp 483–512 Google Scholar Ebert TA, Dixon JDD, Schroeter SC, Kalvass PE, Richmond NT, Bradbury WA, Woodby DA (1999)...
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