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Using a dental drill, a 3-mm-diameter cranial window was made over the left somatosensory cortex (ML: -3 mm, AP: -2 mm to bregma). The skull was removed and the dura was left intact. A custom-made glass coverslip plug (OD = 5 mm, ID = 3 mm) was placed on top of ...
(Life Technologies) and Sanger sequencing, respectively3,4. However, the NGS sequencing platforms widely used for metagenomic sequencing, such as Ion Torrent and Illumina, require comprehensive pre-sequencing preparation of samples and require the sequencing run to completion before analysis can start (...
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