If the animal failed to find the platform within 1 min, it was physically guided to the platform. The escape latency(s) and path length (cm) to find the platform were measured in each trial and averaged over three trials for each rat. Table 1. Depiction of different starting positions ...
AllergenBiological ActionMol Weight kDaAllergenicity ReferenceFunctional Consequence Der p 1Cysteine protease (papain-like)2497Disruption of tight junctions. Cytokine, chemokine & growth factor production. Eosinophil and mast cell degranulation. Fibroblast maturation & collagen production. ...
verbenone formed two hydrogen bonds with the backbone nitrogen of Leu185 and Ala186, along with Van der Waal interactions with nearby hydrophobic residues, e.g., Leu185 and Tyr244. However, verbenone failed to interact with the active site zinc, which is crucial...
Our previous studies suggest that human fat extract (FE) contains a variety of angiogenic factors and may provide an alternative treatment option for stroke. However, the therapeutic effect is largely limited due to its short half-life, and inaccurate ta
Mice that failed to find the platform within 60 s were removed and placed on the platform for 15 s before being returned to their cages. Videos of the whole process were taped and results interpreted using Graph pad Prism. Rotarod and pole climb test Rotarod and pole tests were used to ...
Briefly, the cells were incubated with the EZ-Cytox solution (containing a water-soluble tetrazolium salt) for 2 h at 37 °C. Cell viability was determined by quantifying the metabolic conversion of the tetrazolium salt to formazan dye. The absorbance of the supernatant was measured at 450 nm...
2.7. Cell Viability Assay Prostate cancer (PC3) cells and normal Vero cells were obtained from Nawah Scientific (Inc., Cairo, Egypt), and about 1 × 105 cells/mL (100 µL/ well) were inoculated in the 96-well tissue culture plate, then incubated at 37 °C, 5% CO2, for 24 h to...
Combined chemotherapy and radiation is meanwhile commonly used for advanced head and neck cancer in order to preserve laryngeal and/or pharyngeal structures. Paclitaxel is one of the agents used with high response rates, however it failed to reach a local-regional tumour control in 12% of patients...
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A significant reduction of ROS formation in both cell types after pretreatment with BNO 1095 for 1 h prior to stimulation with PMA was observed. The cell-free DPPH radical scavenging assay demonstrated anti-oxidant activity of the BNO 1095 extract, with an IC50 value of 92.3 μg/ml (Fig. ...