In the Synthase-dependent pathway (Fig. 1, right), the polysaccharide is simultaneously polymerized and exported across the plasma membrane by a synthase, Alg8 or BcsA in alginate or cellulose production, r
While EPS biosynthesis pathways are central to biofilm formation, their distinct contributions and regulatory dynamics remain incompletely understood. Here, we systematically dissect the roles of three core EPS pathways—Psl, Pel, and alginate—in biofilm architecture and function using multi-o...
In the presence of physiological concentrations of Ca2+, and both eDNA and the polysaccharide alginate must be removed from P. aeruginosa biofilms for successful phagocytosis by PMN (Wells et al., 2023). Removing eDNA or alginate on its own does not increase the success of phagocytosis, and ...
Panel C is a schematic representation of the main components of the biofilm matrix and their functions. The biofilm matrix consists of a wide array of functional biomolecules such as exopolysaccharides (Pel, Psl, alginate, Vibrio polysaccharide (VPS), β-glucans, α-mannans, polysaccharide ...
The ECM is the structure that binds cells in biofilms, acting mainly as a scaffold of these three-dimensional structures. It is composed mostly of different EPSs, extracellular proteins, and DNA33. The ECM of F113 is predicted to contain alginate, levan,Pseudomonasacidic polysaccharide (Pap), an...
It is composed mostly of different EPSs, extracellular proteins, and D NA33. The ECM of F113 is pre- dicted to contain alginate, levan, Pseudomonas acidic polysaccharide (Pap), and poly-β-1, 6-N-acetylglucosamine (PNAG) polysaccharides, functional amyloids in Pseudomonas (Fap), large ...
Furthermore, I demonstrated that Pel and Psl polysaccharides provide structural redundancy in the biofilm matrix and the structural contribution of each polysaccharide was highly variable between clinical and environmental isolates. This led us to propose four classes of strains based upon their Pel and...
PssUMAF0158 contains two additional genomic regions that putatively encode for exopolysaccharides such as alginate and a Psl-like polysaccharide. To date, the Psl polysaccharide has only been studied in Pseudomonas aeruginosa, in which it plays an important role during biofilm development. However, ...
aeruginosa strains, PAO1 and PA14.; We hypothesized that there are alternative polysaccharides involved in mediating the initiation of biofilm formation. One putative polysaccharide locus, designated psl (p&barbelow;olysaccharide s&barbelow;ynthesis l&barbelow;ocus), was derived from the annotated ...
the sizes of colonies of an EPS−mutant (ΔvpsL; a gene required for synthesis of the key matrix polysaccharide)23do not depend on the agar concentration. These findings demonstrate that EPS production is essential forV. choleraecolony biofilms to expand differentially in response to changes in...