The extracellular matrix (ECM) is made up of a complex macromolecular network of proteins and proteoglycans providing both rigidity and flexibility to the tissue structure of all organs. However, beyond acting as a structural support for the cells, the ECM also has important regulatory functions, ...
b), a finding that is in agreement with previous observations31. Blood clots are not only composed of a fibrin matrix, but also include blood-borne cells, such as platelets
The extracellular matrix (ECM), a complex meshwork of proteins, is a fundamental component of multicellular organisms. The ECM is the three-dimensional architectural scaffold that defines tissue boundaries and biomechanical properties [1] and cell polarity. It also serves as an adhesive substrate for...
We examined differences in the differentiation of male and female hPSCs into pSMCs, and investigated the effect of 17β-estradiol (E2) on the extracellular matrix (ECM) metabolisms and cell proliferation rates of the pSMCs. Statistical analyses were performed by using Student’s t test or two...
CaSki cells contain around 600 copies of HPV 16 virus in the genome, whereas SiHa have only 1–2 copies per cell. Cells were analyzed using two different human tumor derived extracellular matrix methods (3D myoma disc model, and Myogel Transwell invasion assay). Cultures were irradiated with ...
The extracellular matrix is a complex, dynamic and critical component of all tissues. It functions as a scaffold for tissue morphogenesis, provides cues for cell proliferation and differentiation, promotes the maintenance of differentiated tissues and enhances the repair response after injury. Various amo...
The ability to recreate an optimal cellular microenvironment is critical to understand neuronal behavior and functionality in vitro. An organized neural extracellular matrix (nECM) promotes neural cell adhesion, proliferation and differentiation. Here, we expanded previous observations on the ability of nEC...
syringe. This can be followed by the addition of buffer to wet the filter and prevent air bubbles. Subsequently, the required volume of gel filtration matrix can be added, filled with elution buffer, wetted, and finally used (Fig.2c) [73]. Foers et al. used SEC for EVs isolation from...
found the release of oncosomes from amoeboid-like invasive tumor cell, which is facilitated by soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) protein and vesicle-associated membrane protein (VAMP) with cargo delivery of membrane-type 1 matrix metalloprotease (MT1-MMP) [...
Biofilms, surface-attached communities of bacteria encased in an extracellular matrix, are a major mode of bacterial life. How the material properties of the matrix contribute to biofilm growth and robustness is largely unexplored, in particular in respo