Here, we show that two members of the extended synaptotagmin (E-Syt) family, E-Syt1, and the short isoform of E-Syt2 (E-Syt2S), contribute to activation of CRAC channels in T cells. Knockdown or deletion of bothandreduced store-operated Caentry (SOCE) and ORAI1-STIM1 clustering in...
世联博研公司现货供应abbexa品牌订货号为Catalogue No: abx335644的Extended Synaptotagmin 2 (ESYT2) Antibody (FITC)产品
2. E-Syts function as ER-PM tethers Synaptotagmins are a family of intrinsicmembrane proteinsconcentrated on secretory vesicles, including synaptic vesicles. They comprise a N-terminaltransmembrane domain(with the N-terminus in the vesicle lumen) and two cytosolic C2 domains[1],[10]. They are ...
Here, we show that the three extended synaptotagmins (E-Syts) are ER proteins that participate in such tethering function via C2 domain-dependent interactions with the PM that require PI(4,5)P2 in the case of E-Syt2 and E-Syt3 and also elevation of cytosolic Ca2+ in the case of E-...
we demonstrate that peroxisomes form membrane contacts with the ER through the interaction between peroxisomal PI(4,5)P2and ER-resident extended synaptotagmin-1, 2 and 3 (E-Syts). Depletion of peroxisomal PI(4,5)P2or E-Syts markedly decreases peroxisome-ER membrane contacts and induces choleste...
There are three E-Syts in mammals, which all contain an N-terminal transmembrane (TM) hairpin for the association with the ER membrane, and a central synaptotagmin-like mitochondrial-lipid binding protein (SMP) domain as a carrier for phospholipids. Additionally, mammalian E-Syt1 and E-Syt2/...
We present at 2.44 Å resolution the crystal structure of a fragment of human extended synaptotagmin 2 (E-SYT2), including an SMP domain and two adjacent C2 domains. The SMP domain has a β-barrel structure like protein modules in the tubular-lipid-binding (TULIP) superfamily. It dimerizes...
(4,5)P2 and ER-resident extended synaptotagmin-1,2 and 3(E-Syts).Depletion of peroxisomal PI(4,5)P2 or ESyts markedly decreases peroxisome-ER membrane contacts and induces cholesterol accumulation in lysosomes.Furthermore,we show that cholesterol is delivered from 3H-labeled peroxisomes or PI(...
The assembled t-SNAREs in turn provide efficient binding sites for the Ca2+ sensor synaptotagmin 1 (Syt1), which is localized on synaptic vesicles and thus can bridge the two membranes (45). Indeed, cellular and in vitro assays demonstrate that Syt1 docks vesicles in a Ca2+-independent ...
Extended synaptotagmins (E-Syts) mediate lipid exchange between the endoplasmic reticulum (ER) and the plasma membrane (PM). Anchored on ER, E-Syts bind the PM via an array of C2 domains in a Ca2+- and lipid-dependent manner, drawing the two membranes close to facilitate lipid exchange....