DNA parts and plasmids used in this study are listed in the supplementary materials.E. coliTurbo (NEB) cells were used for plasmid construction. Thetyr1DNA sequence was ordered from Twist Bioscience, with compatible 3′ and 5′ overhangs for entry into the KTK via Golden Gate Cloning. Constit...
The nourseothricin acetyltransferase marker was finally removed by transient expression of aCre-recombinase. This gene was cloned into a plasmid with an autonomously replicating sequence44and a Zeocin-resistance cassette, which was then transformed into the Δhis4strain. The transformants were incubated o...
In the first chapter, I talked a bit about matching HTML tags, such as the sequence <B>very</B> that renders the “very” in bold if the browser can do so. Attempting to match a <B>...</B> sequence seems similar to matching a quoted string, except the “quotes” in this case...
Salmon version 1.3.0 [67] was used to quantify the abundance at the transcript level with the options: (1) sequence-specific bias (–seqBias), (2) fragment-level GC bias (–gcBias), (3) –validateMapping, and (4) appropriate library type (-l ISR). The transcript abundance was quanti...
Primer sequences and probe numbers are described (Supplementary Table SI). Adenoviral Infection The construction of FLI1-expressing and PDEF-expressing adenovirus has been previously described [12], [31]. Cells were infected in normal growth medium using the lowest dilution of either control virus ...
Expression like(java.lang.String value, java.lang.String escapeSequence) PUBLIC: Return an expression that compares if the receivers value is like other value. Expression likeIgnoreCase(Expression theValue) PUBLIC: Return an expression that compares if the receivers value is like the other value, ...
If Squeak had not already defined the method whileTrue:, we could have recursively redefined it in the BlockContext class as: whileTrue: aBlock self value ifTrue: [aBlock value. self whileTrue: aBlock] Please also note that Squeak compiles this version using whileTrue: in a sequence of ...
These complexes are specific to the CD5X probes, as they can not be competed away with the addition of excess irrelevant oligos, CD5Y, CK, and KE (figure 6a, lanes 4-6). Figure 5 a: Sequence comparison of the Ets site, the CD5X region, and the CD5Y region between human and mouse....
Additional file 2:Gene inventory, isoenzyme structural properties, crystallographic data, kinetic constants (native VP1, MnP4 and MnP1, and mutated variants), and sequence identities for different PODs from the P. ostreatus genome. Table S1. Inventory of peroxidase genes in the genomes of P. ost...
The first thing we have to care about is the operator precedence. In any logical expression, several parts must be evaluated in the correct sequence order. For instance, given the expression2 + 3 * 5, we must evaluate the multiplicative expression first or it would result in a wrong value...