Here we describe a new array system (miRCURYTM LNA Array) for global microRNA (miRNA) profiling of biological samples. The miRCURY LNA Array system uses locked nucleic acid (LNATM), a high-affinity nucleotide a
(Idiopathic pulmonary fibrosis[All Fields]AND“Homo sapiens”[porgn]AND(“gse”[Filter]AND(“Expression profiling by array”[Filter]OR“Expression profiling by high throughput sequencing”[Filter])). GEO experiments were manually curated, abstracts and related articles were revised, and only datasets...
Once expressionmicroarrayshave been hybridized and scanned, the fluorescence signal provided by each probe type within each cell of the array is transformed into a numeric value. In order to get an accurate expression value for each specific biological entity tested (gene, transcript, or exon), th...
Fifteen genes that were differentially expressed between the two chicken lines were validated by real-time RT-PCR. To validate the results further, in addition to the samples used in the array experiments, four RNA pools (two from the fat line and two from the lean) were used for real-...
Microarray analysis was carried out by Phalanx Biotech Group using OneArray (array version HOA 6.1) which contains 31,741 mRNA probes that can detect 20, 672 genes in human genome. In brief, the purity of the extracted RNA was checked using NanoDrop ND-1000. The Pass criteria for absorbance...
This study presents a pipeline for endometrial single-cell gene expression profiling. RNA-Seq was used to identify differentially expressed genes in biopsies vs. cultured individual cells. Read Publication Gene Panel and Array Finder Identify sequencing panels or microarrays that target your genes of in...
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Expression profiling of a high-fertility mouse line by microarray analysis and qPCR Download PDF Jens Vanselow, Gerd Nürnberg, Dirk Koczan, Martina Langhammer, Hans-Jürgen Thiesen & Norbert Reinsch 5229 Accesses Explore all metrics ...
Gene expression profiling by DNA microarrays The global gene expression analysis was performed using the Affymetrix GeneChip® Mouse Genome 430 2.0 Array and GeneChip® Expression 3′Amplification reagents (Affymetrix, Santa Clara, CA) according to manufacturer’s protocols if not stated otherwise. Th...
cDNA synthesis in both solution and on the microarray surface. MCF-7 and 3T3 cells were used in further single-cell experiments by smearing them on the microarray surface after fixation43. Before attachment to the array surface, the buffer was exchanged to 0.1 × saline sodium citrate (SSC) ...