The invention provides auto-inducible systems for expressing recombinant proteins of interest which take advantage of elements of quorum sensing (QS) systems of certain bacteria. These systems can be used to produce commercial quantities of proteins such as antigens, which can be used to prepare ...
First, K. lactis has been used to produce proteins at industrial scale in the food industry for over a decade due to its ability to rapidly achieve high culture densities and abundantly produce recombinant proteins (Figure 2). Second, yeast expression is driven by a variant of the strong LAC...
Expression of Proteins in E. coli Expression of a recombinant protein can be approached in general by constructing a plasmid that encodes the desired protein, introducing the plasmid into the required host cell, growing the host cells and inducing protein expression, and then lysing the cells,purif...
A protein’s expression limit, which triggers cellular defects, is a useful indication of the underlying mechanism. In this study, we developed an experimental method of estimating the expression limits of target proteins in the human embryonic kidney cell line HEK293 by measuring the proteins’ ...
PROTOCOL A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag Sreedevi Nallamsetty & David S Waugh Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute at ...
The results indicated that the OPR-like gene most strongly induced by the inoculation of pea plants with a compatible pathogen and by treatment with the suppressor from M. pinodes was PsOPR2. Furthermore, the ability of the six recombinant OPR-like proteins to reduce a model substrate, 2-...
The aim of this study was to overexpress Pv D1 in a prokaryotic system, verify the biologic function of recombinant Pv D1 (Pv D1r) by comparing the antimicrobial activity of Pv D1r to that of the natural defensin, Pv D1, and use a mutant Candida albicans strain that lacks the gene ...
Use of recombinant proteins varies widely - from functional studies in vivo to large-scale protein production for structural studies and therapeutics. Using the best expression system for your protein and application is key to your success. The following table highlights the characteristics of ...
Digestion of the radiolabeled in vitrotranslated proteins with baculovirus-derived recombinant PfSUB-1 (rPfSUB-1) and subsequent analysis by SDS-PAGE and fluorography were as described previously (5). Expression of rPfSUB-1 in Insect Cells Recombinant baculovirus was prepared by recombination between...
We have significant experience in the expression and purification of recombinant proteins, utilizing various protein modalities, including prokaryotic, yeast, insect (baculovirus), and mammalian cell expression systems. In addition, employ fusion technologies to provide our clients with more options for pro...