Here, we investigated the effect of non-formylated methionine-conjugated Escherichia coli tRNAi on the synthesis of the protein initiation of interest (PII) in HeLa cells under intracellular L-methionine levels. We found that E. coli methionine-tRNAi counteracts human methionine-tRNAi, indicating ...
Although metastatic disease is the leading cause of cancer-related deaths, its tumor microenvironment remains poorly characterized due to technical and biospecimen limitations. In this study, we assembled a multi-modal spatial and cellular map of 67 tumor biopsies from 60 patients with metastatic breast...
Briefly, small RNA fragments were purified by acrylamide gel purification and 3′ and 5′ adapters were ligated to the ends of single stranded small RNA molecules. Reverse transcription and PCR amplification was performed to generate the DNA colonies template library, which was diluted to 10 nM. ...
enriched terms across differentially expressed proteins generated by Enrichr using the Bioplanet 2019 library (see DataS3). The significance of the term is defined on the x-axis. Terms of interest are emboldened.P-values were computed using a two-sided Fischer’s exact test.f–jLipid droplets ...
All organisms of life are dependent on the continuous sensing of their environment. These sensing machineries, including one-component regulatory systems, enable the cell to constantly perceive the extra- and intracellular status and to translate changes directly into altered gene expression allowing a ...
B: Reflection of AIBS gene expression profiles sampled in the right hemisphere (RH, yellow dots) onto the LH (red dots) to increase sample density. C: Regional gene expression profiles from the AIBS Human Brain Atlas were mapped to the same cortical parcellation template to estimate regional ...
Of particular interest was the finding that the basal level of IFN-γ in the spleen was lower in the SS+S group of HIV-1Tg rats than of the F344 rats, but was significantly increased in the SS+L and LL+L groups of the HIV-1Tg rats compared to the F344 rats (Table 3). A recen...
Of particular interest was the finding that the basal level of IFN-γ in the spleen was lower in the SS+S group of HIV-1Tg rats than of the F344 rats, but was significantly increased in the SS+L and LL+L groups of the HIV-1Tg rats compared to the F344 rats (Table 3). A recen...
One solution could be to cluster profiles prior to applying DynOmics, to identify specific patterns of interest over time as queries and/or references. As the algorithm is based on independent pairwise comparisons, parallel computing could also be used to decrease the computational burden. ...
H2O was used as template for the control. (D) Cloning of full-length NBCn2 derived from promoter P2 by nested RT-PCR with rat kidney. (E) Western blotting of the distribution of NBCn2 proteins in cellular fractions of rat kidney homogenate. of ~150 bp was obtained. The 5′ -RACE...