2001). Isolation of genes coding for fungal transcription factors and for example, their subsequent overexpression in fungal hosts opens up a new route for industrial strain modification. Expression cloning is also making possible to discover enzyme genes originating from organisms which have not been ...
regions detected by centromere-specific repetitive sequences.d, Distribution of insertion time of full-length Copia and Gypsy LTRs in the 27 individual genomes. Each line represents a genome in the left graph. The two circles show the Copia and Gypsy LTRs that can be clustered into groups with...
Coding sequences (CDS) were retrieved from the Ensembl database. The longest transcript was retained when multiple transcripts were annotated to the gene. The quality of gene sets was evaluated using BUSCO 4.0.563 with the single-copy ortholog set vertebrata_odb10 (Supplementary Fig. 8). Transcr...
(http://www.huber.embl.de/users/anders/HTSeq/doc/count.html). Reads assigned to annotatedcoding regionsof the genome will be counted based on different assumptions, depending on the used method. In order to reduce deviations in the abundance estimation per gene or transcript, two normalization...
Que Q, Wang H-Y, English JJ, Jorgensen RA (1997) The frequency and degree of cosuppression by sense chalcone synthase transgenes are dependent on transgene promoter strength and are reduced by premature nonsense codons in the transgene coding sequence. Plant Cell 9:1357–1368 ...
Any reference to expression_name in the query uses the common table expression and not the base object. column_name Specifies a column name in the common table expression. Duplicate names within a single CTE definition aren't allowed. The number of column names specified must match the number ...
The simplest definition of cis-eQTLs versus trans, refers to genetic variants that affect expression in an allele specific manner, with implications on underlying mechanism. Yet, due to technical limitations of expression microarrays, the vast majority o
Nevertheless, it can be argued that this definition is conceptually simpler and, more importantly, easier to reason about in the context of complex expression. Regarding practical uses, looped lookarounds like the one in the example are quite rare anyway. Internals DFA construction The library ...
coding gene expression after sequencing as previously reported by [8], while data sequenced using InDrop, Cel-Seq, or Cel-Seq2 shows prominent ribosomal protein coding gene expression (Additional file1: Fig. S8D, left panel). When reconstructing smartseq2-hq data with indrop-lq data as ...
The molecular responses activated by heat or by unfolded protein accumulation have been studied extensively in separate contexts [17,80,97–99]. However, both responses converge in the activation of genes coding for chaperones and proteases. For instance, some genes (such asdnaK,dnaJ,ibpA,ibpB,...