Antifreeze proteins (AFP) are important for freezing point depression in 2nd instar Choristonettra fumiferana, spruce budworm larvae. A total of 14 sequences encoding several AFP isoforms were cloned and characterized. The importance of specific residues in the structure and function of C. fumiferana...
Aliquots of urine samples were centrifuged at 10,000 × g for five minutes to remove suspended material, if any, and the supernatants were used to measure the osmolality by freezing point depression using a Microosmometer Model 3300 (Advanced Instruments Inc., Norwood, MA, USA). Assay of ...
Plasma osmolality was measured by freezing point depression on an Advanced Digimatic osmom- eter (model 3MO, Advanced Instruments, Needham Heights, MA, U.S.A.). Plasma sodium, potassium, and chloride concen- trations were determined by a Nova 5 electrolyte analyzer (Nova Biomedical, Waltham, ...
in the forced swim test (FST) of the naı¨ve, unstressed Blood samples were collected for the determination of animals; therefore, WMIs represent an endogenous plasma corticosterone (CORT) levels and adrenal depression model.33 The chronic stress model em- weight was also determined. The ...
mice were placed in an altered context chamber modified by new floor and side inserts. Mice were measured for freezing during an initial 3-min period and during a 3 min presentation of the CS. Freezing was defined as the cessation of movement other than respiration for ≥750 milliseconds. ...
transfer your sense of how the lenses we visually use, can enhance, limit, or filter what we take in of the worlds we inhabit. I certainly have a viewpoint, and it may or may not be clear what I am up to here. Please take a look if you are up for a technological “adventure....
Childhood maltreatment represents a major risk factor for the development of numerous childhood psychopathologies that in many cases linger as chronic mental illnesses in adulthood. Exposing rodents or non-human primates to early life stress increases an
“I respond to intensity, but I also like reflection to follow action, for then understanding is born, and understanding prepares me for the next act.” JANUARY SNOW, ARTIC BLAST, ICE, FREEZING. Maelstrom of inconveniences toppling down in every nook and cranny of body, home, and outdoors....
At the start of the treatment, brains were moved into either new PBS (no treatment) or PBS with 700 μM ActD (treatment) for the duration. Brains were kept on a nutator during treatment. At the end of the treatment, brains were flash-frozen. After freezing, RNA was prepared and the...
Male germ cell development requires precise regulation of gene activity in a cell-type and stage-specific manner, with perturbations in gene expression during spermatogenesis associated with infertility. Here, we use steady-state, nascent and single-cell