To analyze the expression of Pcdh-a3, Pcdh-a5, and Pcdh-a7 in the dup(2-c2) allele, single Purkinje cells from 4-week-old PcdhaJF1/dup(2-c2) mice were picked up by glass capillary. Complementary DNA of Pcdh-a3, Pcdh-a5, Pcdh-a7, and Pcp-2 (a marker for Purkinje cells) was ...
Ledley et al., “Retroviral Gene Transfer into Primary Hepatocytes: Implications for Genetic Therapy of Liver-Specific Functions,” Proc. Natl. Acad. Sci. USA 84(15):5335-5339, Aug. 1987. Lim et al., “Long-Term Expression of Human Adenosine Deaminase in Mice Transplanted with Retro-Virus...
While the number of sequences available for bivalves has increased dramatically during the past few years [40–43], very few genes are either fully annotated and/or functionally characterised, often leading to a mean proportion of unknown sequences higher than 50%. In a bivalve such asB. azor...
We set these criteria: missing data were not acceptable, the lowest Cq in Dynamic Array should be 6 and three samples should be distinguished and fall into respective groups. That is why, the two lowest concentrations (1.25 ng and 5 ng) for 15 cycle pre-amplification were removed from the...