DNA sequences of the resulting 27 peaks and 63 previously identified GIs were extracted from the mm10 genome and subjected to the XSTREME online server (https://meme-suite.org/meme/tools/xstreme) for de novo motif discovery with default parameters. The resulting motif with the most significant...
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The homogenate was collected in a centrifuge tube and kept on ice for 30 minutes followed by centrifugation at 800 g for 15 min. The supernatant was collected, and centrifugation was repeated to isolate mitochondrial and cytosolic fractions. The supernatant was then centrifuged at 11,000...
stacked disc centrifuge at 600 mL/min and 12,000 relative centrifugal force (RCF) to separate inclusion bodies from suspended cell debris and dissolved impurities. The recovered paste was re-suspended at 15 g/L (dry basis) in water and the pH adjusted to about 10.0 using NaOH. The ...
The separation of PBMC was carried out using Ficoll separation medium with density 1.077 g/L and LeucoSep™ centrifuge separation tubes (by GreinerBioOne, Austria) containing porous barrier which enable cell separation by means of density gradient centrifugation and purification following the ...
Centrifuge the sonicated mixture at 7068× g for 25 min at 4 °C and discard the supernatant. Note: The sonicated mixture was centrifuged at 7068× g based on the maximum speed allowed on our centrifuge...
82 4 of 20 ethyl esters and other esters were significantly increased and the contents of alcohols, al- dehydes and ketones were significantly decreased in the red stage of 'Sachinoka' and its 4 of 19 somaclonal mutant 'Mixue' fruits compared with the first three stages of fruit develop- ...
The SCF was cut into small pieces and transferred into 50 ml centrifuge tubes. To extract the stromal vascular cells from the tissue matrix, an enzymatic digestion was done with a digestion medium (DMEM/F12 with 15 mM NaHCO3, 100 mM HEPES (Carl Roth), 1.5 % BSA (Sigma-Aldrich) and 2...
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and 3 ml of 1× ST buffer was added to a total well volume of 5 ml. The solution was centrifuged in a 15-ml Eppendorf tube for 5 min at 500gand 4 °C in a swinging bucket centrifuge. Pellet was resuspended in 1× ST buffer with a resuspension volume of 100–200 μl...