The solution was centrifuged in a 15-ml Eppendorf tube for 5 min at 500g and 4 °C in a swinging bucket centrifuge. Pellet was resuspended in 1× ST buffer with a resuspension volume of 100–200 μl based on pellet size. The single-nucleus suspension was filtered through a 35-...
Cells were collected by centrifuge at 350g for 5 min and resuspended in 1× PBS. All cell lines were maintained at 37 °C with 5% CO2 at a recommended density. Dissociation of single cells from the mouse olfactory epithelium The MOE was dissected and minced into small pieces, then ...
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The separation of PBMC was carried out using Ficoll separation medium with density 1.077 g/L and LeucoSep™ centrifuge separation tubes (by GreinerBioOne, Austria) containing porous barrier which enable cell separation by means of density gradient centrifugation and purification following the ...
The homogenate was collected in a centrifuge tube and kept on ice for 30 minutes followed by centrifugation at 800 g for 15 min. The supernatant was collected, and centrifugation was repeated to isolate mitochondrial and cytosolic fractions. The supernatant was then centrifuged at 11,000...
for 3 min onto poly-lysine- coated slides using a Shandon Cytospin 4 Cytocentrifuge (Thermo Fisher Scientific, MA, USA). Slides were immediately fixed using a spray fixative (Shandon Cell-Fixx, Thermo Fisher Scientific) and then processed for immunostaining as above described. Fluorescence was ...
PDLFs were either conventionally seeded or embedded into different 3D structures (spheroids, Mebiol® gel, 3D scaffolds) and exposed to compressive force or incubated without pressure. For one 3D scaffold (POR), we also tested the effect of different compressive forces and application times. ...
Renal hypoxia and its associated oxidative stress is a common pathway for the development of kidney diseases, and using dietary antioxidants such as flavan-3-ols to prevent kidney failure has received much attention. This study investigates the molecular mechanism by which flavan-3-ols prevent hypoxi...
tetrazolium (NBT, Cayman Chemicals, Michigan, USA) perfusion test was performed to localize the de novo ROS production in the kidney [19]. The details about the localization of superoxide generation (blue formazan particles) in the kidney are presented inSupplementary Materials....
Background: Series of whole-body cryotherapy (WBC) among healthy and physically active individuals can potentially reduce inflammatory response, although exact mechanisms remain unclear. Methods: The impact of whole-body cryotherapy on inflammation modul