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/usr/include/c++/7/bits/vector.tcc:95:7: note: candidate: void std::vector<_Tp, _Alloc>::emplace_back(_Args&& ...) [with _Args = {}; _Tp = tsecer; _Alloc = std::allocator<tsecer>] vector<_Tp, _Alloc>:: ^~~~ /usr/include/c++/7/bits/vector.tcc:95:7: note: candidate ...
It uses Simple Good-Turing smoothing to provide a non-zero model estimate for genes that were not observed in the representative empty GEM set. Finally, the RNA profile of each barcode not called as a cell in the first step is compared to the background model. Barcodes whose RNA profile ...
To process this query, we perform a bitwise AND on these two vectors, which produces the results vector: (0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0). The query returns the set of documents that correspond to the 1s in the results vector, which is {d13}. For the OR operator...
MsAA NTag; lane 4: cell-free extract with MsAA CTag; lane 5: insoluble fraction with MsAA CTag; lane 6: cell-free extract with MsAA noTag; lane 7: insoluble fraction with MsAA noTag; lane 8: cell-free extract of empty vector control; lane 9: insoluble fraction of empty vector control...
(Eppendorf). Twenty-four hours after seeding, cells were transfected with 50 ng per well of either reporter plasmid pMIR-RNL-TK, with or without insert, and 200 ng per well of miRNA expression plasmid pSG5-miR-29a or the empty expression vector pSG5. Forty-eight hours post-...
(GEM). Additionally, cells were equipped with an inducibleCrerecombinase expression plasmid or an empty vector (control). To determine the optimal induction time for the Cre recombinase, we tested various induction periods with cells bearing the 5’ GEM and observed that expression variation was ...
1.13.4.3The Contribution of Vector and Host Cell For recombinant cell lines producing biologics, productivity is a function of both the host cell background and the transgeneexpression vector[13]. Expression vectors are designed for maximum transcription through selection of efficient promoters, enhancers...
vector<expression_t> expression_list; parser_t parser; expression_t expression; symbol_table_t symbol_table; expression.register_symbol_table(symbol_table); for (std::size_t i = 0; i < 3; ++i) { if (parser.compile(expression_str[i],expression)) { expression_list.push_back(expression)...
The PTHLH and EMT-promoting SNAI2 mRNA levels in the cells ectopically overexpressing TCF4 or TWIST1 alone was approximately twofold higher than those in cells infected and transfected with green fluorescence protein (GFP)-expressing adenovirus and empty vector (Figure 2H,I). Compared with those in...