lipolytica has been accorded a GRAS (generally recognized as safe) status [19]. When developing an efficient cell factory, the choice of the promoter driving recombinant gene expression is crucial, and therefore represents one of the key parameters to be optimized. At present, few promoters have...
In this study are described the cloning, expression, purification and spectroscopic characterization of a new Fe–S cluster-containing protein, a putative HdrC homologue, from A. ferrooxidans strain LR. The oligomeric state of the protein was assessed by both dynamic light scattering and size-...
(11), 2024 – 2031 & 2005 Cancer Research UK Reduced CCL2 expression in ovarian cancer JM Arnold et al HOSE1H7O.1SEP1E.1O1OAW4J2AM HEY SKOVC3OLOO31V6CAORV3CAORV4CAORV5CAORV8CAO42V0CAO4V32CAP4E3O31247/87A2780OVHSD-1OV13 A CCL2 0.7 kb 2027 B GAPDH 1.3 kb Figure 2 ...
Immunoprecipitation was carried out using either 2 μg of anti-SRF antibody (Cell Signaling; catalog no. 5147), 5 μg of anti-MRTF A antibody (Santa Cruz Biotechnology; catalog no. sc-32909), or normal rabbit IgG (Santa Cruz Biotechnology; catalog no. sc-2027) as a negative control. ...
Cell lysates were precleared sequentially with normal rabbit IgG (sc-2027, Santa Cruz Biotechnology) and protein A-agarose. Anti-AR antibody from Santa Cruz (2.0 μg) was added to the cell lysates and incubated at 4 °C overnight. IgG was used as the negative control. Specific primer...
Cancer Epidemiol Biomarkers Prev 23(10): 2027–2031. This work is published under the standard license to publish agree- ment. After 12 months the work will become freely available and the license terms will switch to a Creative Commons Attribution- NonCommercial-Share Alike 4.0 Unported License...
Images confirmed the rapid blood clearance of the radiola- beled conjugate, shown by the low background radioactivity. Discussion Earlier, radiolabelled anti-HER3 antibodies, their fragments, and a natural ligand, heregulin, were pro- posed for HER3 imaging using SPECT and PET modalities18,34,...
(BMS)1, DES reduces in-stent restenosis through the local release of anti-proliferative agents to inhibit neointima formation. However, the use of DES in some patients is associated with a risk for adverse effects such as delayed arterial healing and poor re-endothelialization. Moreover, numerous...
2008, 153, 2027–2039. [Google Scholar] [CrossRef] Huhti, L.; Blazevic, V.; Nurminen, K.; Koho, T.; Hytönen, V.P.; Vesikari, T. A comparison of methods for purification and concentration of norovirus GII-4 capsid virus-like particles. Arch. Virol. 2010, 155, 1855–1858. [...
The C-terminal protoxin domain of Bacillus thuringiensis Cry1Ab toxin has a functional role in binding to GPI-anchored receptors in the insect midgut. J. Biol. Chem. 2018, 293, 20263–20272. [CrossRef] 10. Oppert, B.; Kramer, K.J.; Beeman, R.W.; Johnson, D.; McGaughey, W.H....