(10 µg) were separated on the 4–6% SDS-polyacrylamide gels and transferred onto 0.2 μM pore size polyvinylidene fluoride membranes (both from Bio-Rad, Hercules, CA, USA), which were blocked for 1 h with a blocking buffer consisting of 5 % bovine serum albumin (BSA) in 1x TBS (...
To calculate the mean standardised biomarker responses, transformed data was standardised according to the general mean and SDs of the data set. Biomarker deviation indices were calculated by subtracting the mean reference biomarker data from the mean standardised biomarker responses for each concentration...
can be easily ingested by marine life and enter mammals through the food chain. Our previous study demonstrated that following acute exposure to MPs, the serum testosterone content reduced and sperm quality declined, resulting in male reproductive dysfunction in mice. However, the toxic effect of lo...
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Farmers from South Asian countries spray insecticides without protective gear, which leads to insecticide exposure through dermal and nasal routes. Acetylcholinesterase plays a crucial role in controlling neuromuscular function. Organophosphate and carba
This work was supported by a grant from the National Cancer Institute of Canada (with funds from the Canadian Cancer Society and the Terry Fox Run) to RD. RD holds the Canada Research Chair in “Genetics, Mutagenesis and Cancer.” SUPPLEMENTARY MATERIAL Supplementary material is linked to the ...
As a response to DNA damage, XPC mice lungs exhibit increased γ-H2AX, followed by severe atypical hyperplasia. Emissions from vehicles are composed of hazardous substances, with polycyclic aromatic hydrocarbons (PAHs) and metals being most frequently cited as the major contributors to negative health...
(b) Evolution of urbanization in the Bisagno basin over the examined time periods compared with areas exposed to floods and landslides/DSGSDs. A morphometric analysis of urbanized surfaces was completed to show how these areas have changed over the study periods (building surfaces on altimetry, ...
Forty micrograms of protein lysate were separated using 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to PVDF membranes. Afterward, the PVDF membrane was blocked with 5% nonfat milk (Sigma-Aldrich), followed by incubation with primary antibody (diluted according to the ...
International Journal of Environmental Research and Public Health Article Environmental Co-Exposure to Lead and Manganese and Intellectual Deficit in School-Aged Children José A. Menezes-Filho 1,* , Chrissie F. Carvalho 2 , Juliana L. G. Rodrigues 1, Cecília F. S. Araújo 3, Nathália R. ...