Describe different steps of Sanger's method of nucleotides sequencing. Explain the process of DNA replication. What are the main points or most important steps in the process? The function of RNA to DNA include the replication, transcription, and translation. Explain these three processes. Describ...
How would a DNA sequencing reaction be affected if the ratio of ddNTPs to dNTPs were increased? How is CRISPR-Cas used to introduce specific changes into DNA sequences? Describe the Sanger method of DNA sequencing. (a) Explain the difference between sequencing by ...
number of contigs, N50, N count and the Open Reading Frame (ORF) number. ORF calling was performed using Prodigal55and the genome statistics were obtained using the assembly-stats tool (https://github.com/sanger-pathogens/assembly-stats). For each of these parameters, the thresholds for the ...
In single gene DNA sequencing studies of P. marinus nuclear loci, an interesting pattern of large divergence between haplotypes has been observed (ranging from 1.5% to 3.6% maximal difference, Brown et al., 2004, Reece et al., 1997, Robledo et al., 1999), implying a large effective popul...
These seeds of modern biology spawned amazing new advances in the 20th century, most notably with Francis Crick and James Watson's discovery of the structure of DNA in 1953, and Frederick Sanger's pioneering work on DNA sequencing. But the 20th century saw many other huge advances, from ...