Careful analysis of substrate dependence of NdK-ATPase measured under optimal conditions demonstrates a deviation from that which might be described by the Michaelis-Menten equation (FIG. 1). Such dependence is typical for enzyme from different sources, such as ox brain,' dog heart,* dog kidney,...
KM and kcat values were calculated by fitting the data with the Michaelis-Menten equation using GraphPad 6.07 (La Jolla, CA). When it was not possible to measure further, the KM was reported as below the lowest substrate concentration for which a rate could be determined. Conversions Substrate...
To achieve reliable data for Michaelis Menten enzyme kinetics, why is the initial velocity measured under steady-state conditions? You dribble a basketball while walking on a basketball court. List and describe the pairs of action...
Michaelis&ndashMentenmicrobial biomasssagebrush steppesoil ecologysoil incubationsoil respirationEnhanced soil respiration in response to global warming may substantially increase atmospheric CO2 concentrations above the anthropogenic contribution, depending on the mechanisms underlying the temperature sensitivity of ...