In this paper, we propose an approach to introduce security protocols to a general audience, including children or even scientists from different fields. Its goal is to convey the implicit assumptions of our co
its homolog VAP-B, or ORP3 were silenced by short hairpin RNA (shRNA). It was previously demonstrated that VAP-B, in contrast to VAP-A, does not interact with ORP3 and Rab737. Gene silencing was carried out by transfection and stable HeLa cell lines...
Indeed, clonality, while occurring in mul- tiple, phylogenetically wide-ranging lineages3,8, takes very different forms and functions in different clades, some of which may be constrained differently in their responses to environmental gradients9–11. 1Key Laboratory of Hangzhou City for Ecosystem ...
The total RNA was first extracted using the Pure Link RNA Mini Kit (Invitrogen/Thermo Fisher Scientific, Waltham, MA) and a second extraction was conducted by using a modified version of the TRIzol method in which the centrifugation times and total RNA precipitation protocols were opti- mized....
packet that passes through the gateway, verifying the contents of the packet up through the application layer (layer 7) of the OSI model. These proxies can filter on particular information or specific individual commands in the application protocols the proxies are designed to copy, forward and ...
Discussion We present the first-of-its kind single-cell atlas of human nasal samples that spans the lifespan with important and novel coverage during childhood. Using this resource, we provide important insight into the immunobiology of the respiratory system during aging with implications for ...
“one-fits-all” stimulation protocols, a high prevalence of non-optimal targeting and the resulting numbers of potential low- or non-responders may compromise the sensitivity of such statistical approaches to detect stimulation effects. In contrast, the statistical model proposed here tests for ...
The rarity of females in leadership positions has been an important subject of study in economics research. The existing research on gender inequality has established that important variations exist across time and place and that these differences are pa
a nested polymerase chain reaction (PCR) approach was employed to target the internal transcribed spacer (ITS) region of fungal rDNA. The second ITS (ITS2) region of the rRNA operon was sequenced using the ITS3 (5′-GCATCGATGAAGAACGCAGC-3′) and ITS4 (5′-TCCTCCGCTTA TTGATATGC-3′) ...
The “close-hold” -part of the data from the clinical T-Scan examination protocols (the right and left lateral excursions, and the Multi-Bite-procedure21) was manually extracted, frame-by-frame, from the recorded T-Scan movie into a spreadsheet. The three repeated recordings/subject, included...