Fig. 2 Molecular mechanism of type I-D CRISPR-CAS adaptation system CRISPR-Cas gene-editing technology has been widely used in laboratories worldwide. A deeper understanding of CRISPR-Cas system not only broadens the horizon of the human knowledge, but also facilitates developing a new generation ...
How is CRISPR-Cas used to introduce specific changes into DNA sequences? Describe the Sanger method of DNA sequencing. (a) Explain the difference between sequencing by chain termination and sequencing by synthesis. (b) Which one does dideoxy sequencing use? (c) Whi...
However, some patients taking PARP inhibitors develop resistance to the drugs—and some patients do not even respond from the outset. To understand why this should be the case, Professor Steve Jackson and colleagues used cutting-edge CRISPR-Cas 9 gene editing techniques to screenbreast cancer cells...