During host infection, Toxoplasma gondii and related unicellular parasites move using gliding, which differs fundamentally from other known mechanisms of eukaryotic cell motility. Gliding is thought to be powered by a thin layer of flowing filamentous (F
Briefly, 160 simulated trials were run for the SS1, CW, and CCW conditions and 320 simulations were run for the Unique condition, with random noise added to each simulated run. Model parameters were iteratively modified across model runs in an attempt to match the behavioral data as closely ...
Briefly explain the purpose of the loop, or iteration, structure. Then provide an original example algorithm with the loop structure. What are stdin, stdout, and stderr, and how would a programmer use them? Write a program called...
Briefly explain the effects of exercise on pulmonary volumes and capacities. Explain the effect of (1) hyperventilation and (2) holding one's breath on blood pH. Why is the pressure in the pulmonary circulation lower that the systematic circulation? Describe how blood PO2 and PCO2 differ in ...
Briefly, 10 ng of template RNA was reverse transcribed using miRNA-specific stem-loop primers for 30 min at 16 °C, 30 min at 42 °C and 5 min at 85 °C. The PCR reactions were run on ABI Prism 7900HT Sequence Detection System (Applied Biosystems) at 95 °C for...
Briefly, pMHC was coated onto streptavidin-conjugated glass beads via biotin-streptavidin coupling. A pMHC-coupled bead was attached to a biotinylated RBC aspirated on a glass micropipette to form a force probe to test binding with a primary T cell or hybridoma expressing the specific WT or MT ...
All data described so far involving the VOR complex-dependent pathway for the nuclear transfer of viral components refer to HIV-1 pseudotyped with VSV-G. To obtain information about the native virus, we employed p89.6 ΔE ΔN SF-EGFP pseudotyped with autologous HIV 89.6 Env (abbr. HIV-89.6...
Briefly, we first transduced 10–18-week-old WT mice and Tmprss2-KO mice with 2.5 × 109 PFU of FLAG-tagged Ad-ACE2 adenovirus. Consistent with previous findings, predominant ACE2 expression was observed in the alveolar epithelium, as indicated by FLAG staining (Supplementary Fig. 1d)....
We performed chromosome spreading as described previously55. Briefly, GV oocytes were released from milrinone arrest and matured to 5 h after NEBD. The zona pellucida was removed by brief treatment in acid Tyrode’s solution and oocytes transferred to 5 μL of chromosome spreading solution (...
Briefly, Affymetrix GeneChip Human Exon 1.0 ST arrays were analyzed using student’s t-test, assuming unequal variance. The arrays were validated by qRT-PCR for 11 genes7. Data are expressed as mean and percent area positive staining ±SD for gene and protein expression, respectively. P <...