It is possible to improve the resolution of the depth within the framework of the destructive layer-by-layer analysis method by using deconvolution algorithm for the construction of the concentration profiles (in depth) such as the Van Cittert's iterative approach of inverse Fourier transformation....
The raw data were pre-processed into an amplicon sequence variant (ASV) table using our in-house pipeline56 based on the DADA2 algorithm and settings recommended for IonTorrent reads57, with taxonomy assigned to the ASVs using the Ribosomal Database Project (RDP, v18). The resulting ASV ...