trachomatis L2 and D is owing to a frameshift mutation. These findings provide one possible explanation, on a molecular basis, for the differences in susceptibility towards IFN-γ among human serovars of C. trachomatis. 2. Materials and Methods 2.1. Bacteria and host cells HeLa 229 cells and ...
Explain the structural and functional effects that this frameshift mutation (the delta F508 mutation) has on lung cells. Compare and contrast spontaneous and induced mutations, and differentiate between physical and chemical mutagens. Define a ...
We further separated pLoF variants into single nucleotide variants (SNVs) and frameshift insertions or deletions (indels). For our main analyses, we considered all pLoF variants together; pLoF SNVs were used as a sensitivity analysis to ensure patterns were not driven by indels spanning ...
). The c.4253+43G>A mutant yielded bands of 495 and 370 bp, the first corresponding to the correctly spliced product, and the second to a product in which exon 28 was skipped (Fig. 1b, Figure S2). This 125-nt deletion is predicted to lead to a frameshift, and although both WT...
Explain why the deletion of a single nucleotide in a gene (a frameshift mutation) generally results in more severe defects in the polypeptide than a point mutation that just changes a single nucleotide. The Huntington's gene contains Trinucleotide re...
Sec23a+/fl mice were crossed to EIIA-Cre transgenic mice to excise exon 3, resulting in a null Sec23a allele (Sec23a−) with a frameshift and an early stop codon. Sec23a+/− mice were Scientific Reports | 6:27802 | DOI: 10.1038/srep27802 5 www.nature.com/scientificreports/ Figure...
ERAla87 activity can be increased, decreased or remain unchanged comparatively to ERWT. Additionally, the mutation affects the subcellular localization of ER in a cell-type specific manner. How can the differences of ERαA87 functional properties be explained? As previously mentioned, there are ...