To optimize the exosome capture conditions, the number of exosomes before and after capture was measured with BCA to quantitatively calculate the capture efficiency. The Fe3O4@MgSiO3 nanoparticles were modified with CD63 antibodies at different concentrations (0.1, 1, 10, 100, and 1000 µg...
In general, this study presented the variation of miRNAs in blood exosomes before and after radiotherapy. It can not only be helpful for the diagnosis of glioma, but also find new candidate biomarkers for monitoring the condition and evaluating the efficacy of radiotherapy in glioma. It provides ...
Studies have shown that the application of stem cells can achieve follicle regeneration12. Leeet al. confirmed that after MSC transplantation, which can significantly increase the number of ovarian follicles and the oestradiol concentration, mice with chemotherapy-induced POI can maintain long-term ferti...
Methods: In the present investigation, we produced a TBI mouse model and isolated exosomes from their ADSCs before and after an hypoxic pretreatment. We then used next generation sequencing (NGS) to identify differentially expressed circRNAs and luciferase report assays to determine the relationship ...
endocytic pathway [242]. These discrete cellular internalization pathways support the potential therapeutic advantage of exosomes as a drug carrier. However, thorough research is required to understand the cellular uptake of exosomes by specific cell types before developing novel exosome-based cancer ...
Plasma of NSCLC patients with stage IV was also collected before and after anti-PD-1/PD-L1 therapy. Digital PCR was used to detect PD-L1 and 尾-actin mRNA expression of plasma exosomes. Expression of PD-L1 in tumor tissues was determined by immunohistochemical staining (22C3). Pearson ...
MSC-Exos need to be purified and characterized for physical structure and bioactivity function and later pass the product release criteria before being used in clinical trials. The ultracentrifugation method is the most frequently used for isolating MSC-Exos in clinical trials. Suspension components are...
Evaluations were performed before and 2 weeks after immunization. A continuation treatment was performed in 4 cases of non progression. Results The GMP process allowed to harvest about 5 ?? 1014 exosomal MHC class II molecules allowing inclusion of all 15 patients. There was no grade II toxicity...
ImDex were obtained from the culture supernatant of immature DCs derived from donor mouse (C57BL/6) bone marrow and were injected with suboptimal doses of rapamycin into recipient mouse (BALB/c) before and after transplantation. The capacity of this treatment to induce immune tolerance was ...
We defined the optimal exosome dose as approximately 20 μg (per treatment before, during and after transplantation) in rat liver transplantation and the antigen-specific role of Tregs in protecting liver allografts. In the co-treatment group, recipients achieved long-term survival, and tolerance...