The progression of malignancies within patients can be tracked by assessing exosomes content, either before or after anti-cancer therapy, allowing physicians to choose the appropriate treatment option for each individual case [38]. Exosomes separated from the bloodstream of patients with head and neck...
Exosomes have a crucial function in the TME and can act as a diagnostic and prognostic marker for the alteration of TME and the progression of cancer to a certain degree [20]. Their importance stems from their ability to enable intercellular communication and convey a diverse range of micro-mo...
Here we identify that RAB31 marks and controls an ESCRT-independent exosome pathway. Active RAB31, phosphorylated by epidermal growth factor receptor (EGFR), engages flotillin proteins in lipid raft microdomains to drive EGFR entry into MVEs to form ILVs, which is independent of the ESCRT (...
(evidenced by a decrease of their Pearson’s co-localization coefficient) and CD63 eventually accumulates in intracellular compartments while CD9 reaches the cell periphery. However, between 30 min and 1 h after biotin addition, after leaving the Golgi, a fraction of both proteins was found...
Statistical analysis between the conditions (before and after HDL NP treatment) of GFP-SR-B1, integrated normalized intensity and motion was performed using a permutation test45 for means, which does not assume normality of the underlying distributions. Additional Information How to cite this article...
Extracellular vesicles (EV), which are secreted into circulation by many cell types, can provide a snapshot of cellular processes active in disease and healthy cells, allowing the exosomes in circulation to serve as sentinels of the health of an individual. In cancer, exosomes from neoplastic ...
(n = 3; three independent experiments).d, Migration of lung fibroblasts educated with exosomes every other day for 2 weeks was measured as follows. Fibroblasts were plated in 24-well transwell chamber inserts, and after 6 h the number of cells that migrated was counted using haematoxylin ...
After viral adsorption, cells were washed with PBS followed by addition of exosome-depleted media and incubation at 25 °C. The supernatant was harvested and exosomes were isolated as stated above. For analysis of enveloped CrPV, S2 cells were infected for 24 h (MOI 10) before harvesting...
Here we identify that RAB31 marks and controls an ESCRT-independent exosome pathway. Active RAB31, phosphorylated by epidermal growth factor receptor (EGFR), engages flotillin proteins in lipid raft microdomains to drive EGFR entry into MVEs to form ILVs, which is independent of the ESCRT (...