The exon junction complex (EJC) is a dynamic multi-protein complex deposited onto nuclear spliced mRNAs upstream of exon-exon junctions. The four core proteins, eIF4A3, Magoh, Y14 and MLN51, are stably bound to mRNAs during their lifecycle, serving as a
Additionally, the expression level of WRKY genes in defense-related pathways in the shoot apex of PFMAGO- or PFY14-VIGS plants and in the root collar of PFeIF4AIII-VIGS plants was significantly downregulated. Taken together, the Physalis EJC core genes play multiple roles including a conserved...
m6A is highly enriched in the 3’ UTR of a large subset of mature mRNAs to influence mRNA stability and/or translation yet the mechanism responsible for the observed m6A distribution has remained enigmatic. In this study, we find the exon junction complex shapes the m6A landscape by blocking M...
Full function of exon junction complex factor, Rbm8a, is critical for interneuron development Colleen McSweeney, Fengping Dong, Miranda Chen, Jessica Vitale, Li Xu, Nicole Crowley, Bernhard Luscher, Donghua Zou & Yingwei Mao Translational Psychiatry volume 10, Article number: 379 (20...
γ3-Omm Normal A 320 bp internal deletion at V-J4 junction Mutations, deletions, and a 37 bp duplication in the J4-J5 IVS Normal? A large deletion from switch to CH1 leaves its 3′ end Normal γ1-Riv A 80 bp deletion with 56 bp insertion (V 5′ splice site deleted) A 280 bp ...
Genome-wide alternative splicing between panels of normal tissue types have been analyzed previously using a prototype exon microarray (2) and exon junction microarrays (1). Thousands of candidate alternative splicing events were identified, and a subset was RT-PCR-validated with success rates of 86...
(transcript variant expression) or to yield amplicons in exons common to all known transcripts (gene level expression). Assay details are given in additional file4. Where possible, assays were designed to span an exon-exon junction such that contaminations of genomic DNA have no influence. qRT-...
The exon junction complex (EJC), which contains four core components, eukaryotic initiation factor 4AIII (eIF4AIII), MAGO/NASHI (MAGO), Y14/Tsunagi/RNA-binding protein 8A, and Barentsz/Metastatic lymph node 51, is formed in both nucleus and cytoplasm, an
junction in the circRNA produced by the circEIF3J_1 kb plasmid was different from that of circEIF3J, and this difference is shown in the gel image and sequence diagram below. (b) Knockdown of circEIF3J or circPAIP2 with the siRNA resulted in a decrease in the parental gene mRNA level ...
(12% to 15%) of exons showing strong inhibition, enabling more stable mRNAs and flexible protein coding. m6A is majorly responsible for why mRNAs with more exons be more stable. Exon junction complex (EJC) only partially contributes to this exon-intron boundary m6A inhibition in some short ...