Plant Biology Plantlife can be studied at a variety of levels, from the molecular, genetic and biochemical level through organelles, c.. The Origins of Life This tutorial digs into the past to investigate the origins of life. The section is split into geological periods in the.. ...
Molecular biologyBiophysics Designer exons inform a biophysical model for exon definition COLUMBIA UNIVERSITY Lawrence A. Chasin AriasMauricioPre-mRNA molecules in humans contain mostly short internal exons flanked by long introns. To explain the removal of such introns, recognition of the exons instead ...
(snRNPs, see above). Both target one or more of the donor splice site, acceptor splice site or exonic sequences essential for exon definition during pre-mRNA splicing of specific exons. Upon binding of the AON or snRNP to a target exon, the exon will be spliced out with its flanking ...
The molecular mechanisms of exon definition and back-splicing are fundamental unanswered questions in pre-mRNA splicing. Here we report cryo-electron microscopy structures of the yeast spliceosomal E complex assembled on introns, providing a view of the earliest event in the splicing cycle that commits...
[15]. The same concept can also be applied in the laboratory to generate combinatorial protein libraries. Such libraries can be separated in two groups based on the assembly mechanism. Exons can be recombined either at the mRNA level, initiated by self-splicing introns, or post-translationally ...
Altogether, these results strongly suggest that Mago regulates gene expression and exon definition via regulation of Pol II promoter-proximal pausing. Fig. 6 Restoring pausing is sufficient to rescue Mago-associated exon skipping defects. a Mago knockdown results in elevated level of Ser2P ...
The exon junction complex (EJC) is a dynamic multi-protein complex deposited onto nuclear spliced mRNAs upstream of exon-exon junctions. The four core proteins, eIF4A3, Magoh, Y14 and MLN51, are stably bound to mRNAs during their lifecycle, serving as a
Semi-quantitative RT-PCR using GAPDH as a control indicated that the exon-skipped hCDC14B mRNA level was less than half of the wild-type mRNA (Fig. 5c). Such decrease in mRNA level might be due to transcriptional control (altered synthesis) and/or post-transcriptional regulation (altered sta...
Given the heterogeneity of transcriptomes from tumours with and without MYCN amplification, the definition of a perfect relapse signature is still an unresolved task. Nevertheless, the gene- and exon-derived signatures in our study were the only independent markers of survival in multivariate analyses,...
This definition also refers to the complement of a test sequence. Optionally, the identity exists over a region that is at least about 50 nucleotides in length, or more preferably over a region that is 100 to 500 or 1000 or more nucleotides in length. The present invention includes ...