Furthermore, when Erk-2 and a genetic detector are coexpressed in bacteria, the measured R value changes with the substrate sequence with near single residue resolution. Similarly, the cells coexpressing the glycosylating enzyme O-GlcNAc transferase (OGT) and a genetic detector specific for OGT ...
For experiments with VAMP2 and E-Syt1, neurons (1 × 106) were electroporated with 1 μg of DNA using P3 Primary Cell 4D-Nucleofector Kit and the pulse code CU133 on the 4D-Nucleofector System (Lonza Group Ltd., Basel, Switzerland). For experiments with PSD-95, neurons were transfecte...
Supercharged extracellular vesicles, supercharged virus-like particles, and supercharged lentiviral vectors mediate plasmid DNA expression in recipient cells It has been reported that plasmid DNA (pDNA) can be piggybacked into cells with scProteins15. We tested whether EVs associated with both +scProtein...
The NMR structure of human LL-37 (Wang, 2008) is used to map PSSs and define secondary structure element regions, which comprises three distinct parts: NDR (amino-terminal disordered region, residue 1), MHR (middle α-helical region, residues 2–31) and CDR (carboxyl-terminal disordered ...
The germinated seeds were sown in plastic pots (calibre × ground diameter × height = 10 cm × 7 cm × 8 cm, one seed per pot) filled with an organic substrate (vinegar residue: peat: vermiculite = 2:2:1 (by volume), Peilei, Zhenjiang, China), and transferred to the greenhouse. ...
|[gamma]|H2AX: a sensitive molecular marker of DNA damage and repair Phosphorylation of the Ser-139 residue of the histone variant H2AX, forming gammaH2AX, is an early cellular response to the induction of DNA double-strand ... LJ Mah,A El-Osta,TC Karagiannis - 《Leukemia》 被引量: 871...
xylostella using the genomic DNA as a template. The known Z-linked genes PxMasc and Pxkettin were used as positive controls, while the autosomal Pxfl(2)d was used as a negative control. The known autosomal gene defensin was used as the internal reference gene for the analysis of qPCR ...
The coding region may be present in either a cDNA, genomic DNA, or RNA form. When present in a DNA form, the oligonucleotide or polynucleotide may be single-stranded (i.e., the sense strand) or double-stranded. Suitable control elements such as enhancers/promoters, splice junctions, ...
9. An MS2 controlled T7 gene 1 cassette, said cassette comprising a DNA sequence encoding an inducible promoter and the MS2 recognition site each linked to T7 gene 1, and the MS2 coat protein gene under the control of a weak constitutive promoter. 10. A bacterial host cell capable of ...
which uses a DNA fragment comprising a certain known sequence to undergo homologous recombination with a gene with the same or similar sequence in the genome of the recipient cell, so that it integrates into the genome of the recipient cell and is expressed. It ...