140and apoHmp1–189RNCs based on the fluorescence anisotropy decay data shown in this figure and the data in Fig.2. The wild-type (WT) cell-free system, corresponding to the WT strain, was employed for the data shown in this Figure. This cell-free system contains the following chaperone...
we also investigate the effect of the above RNC/r-protein interactions on the bacterial ribosome. The apparent stability of the complexes between RNCs of foldable and intrinsically disordered protein sequences and specific r-proteins is weak and, surprisingly, does not vary significantly with RNC se...
Since pCAG-eGFP or Nanog-eGFP transgenic lines are present in different backgrounds, this phenomenon is allele- and strain-independent. We next sought to delineate the time-frame for paternal genome editing. To do this, we performed single-cell whole genome amp- lification and target locus ...
Since pCAG-eGFP or Nanog-eGFP transgenic lines are present in different backgrounds, this phenomenon is allele- and strain-independent. We next sought to delineate the time-frame for paternal genome editing. To do this, we performed single-cell whole genome amp- lification and target locus ...
529-665-K626W-S631W fragment with RAB6, Myosin II-RBD, and KIF20A-RBD. TheSaccharomyces cerevisiaereporter strain L40 was co-transformed with a plasmid encoding fusion proteins to detect interactions between the KIF20A-RBD-529-665 fragment or KIF20A-RBD-529-665-K626W-S631W fragment and ...
S. aureus strain JLB31 with a plasmid containing a wild-type fakB1 gene, a mutant (pA121I, pA158L), or empty plasmid (pCS119) was inoculated into 5 ml of Luria broth containing 0.1% Brij-58, and tubes were shaken at 37 °C until an A600 of 0.5 was reached. An FA mixture (10...
Fgfr1lox/lox mouse strain This lab N/A Fgfr2Δ5lox/Δ5lox mouse strain This paper N/A Fgfr2Δ8-10lox/Δ8-10lox mouse strain Jackson Laboratory 007569 Fgfr1-T2A-H2B-Cerulean mouse strain This paper N/A Fgfr1-T2A-H2B-GFP mouse strain This paper N/A Fgfr2-T2A-H2B-mCherry mouse strai...
The growth of HFs and CEFs, COS7, CHO, NRK, and RBL cells, infection of cells with the 045 strain of vesicular stomatitis virus and their transfection, stimulation of PC synthesis in HFs (kindly provided by M. De Luca, Istituto Dermatologico dell' Immacolata, Rome, Italy), synchronization ...