IL-13 concentrations in cell-free bronchoalveolar lavage (BAL) as well as IL-4, IL-10, IL-1α, MCP-1, and RANTES in lung homogenates were measured using a Cytokine Fluorescent Bead Immunoassay Assay Kits (Beadlyte®; Millipore, Billerica, MA). Lung homogenates were prepared by using Fast...
Briefly, total RNA extraction from the hippocampi and hypothalami samples was performed using the BioRobot EZ-1 and EZ-1 RNA tissue mini kits (Qiagen GmbH, Hilden, Germany). Then, the purity of the total RNA was examined, and the quantity was estimated using the ND-1000 NanoDrop RNA Assay...