A Human proteome microarray showing potential interacting proteins with Biotin-tagged Drp1 protein. Red arrow indicated the positive control (Biotin-BSA), yellow arrow indicated the negative control (BSA protein
sections were incubated for 1 h with a mouse biotinylated secondary antibody (1:250, Cat. # BA-9200, Vectastain, ABC Elite kit PK-6101, Vector Laboratories) and diluted in blocking solution followed by 1 h incubation with avidin–biotin–peroxidase complex (1:125, Vectastain, ABC Elite kit ...
Then, the samples were replaced by biotin-antibody of PSD-95 and SYN-I and incubated for 60 min at 37 °C. After washing 3 times with PBS, HRP-avidin was added and incubated for 60 min at 37 °C, then, tetra-methylbenzidine substrate was added and incubated for 20 min at 37 °C...
Sections were washed and then incubated in an avidin–biotin horseradish peroxidase ABC solution (Vector Laboratories) in blocking solution for 1 h. Sections were then incubated using a diaminobenzidine substrate kit (Vector Laboratories) according to the manufacturer's instructions and once the reaction...
electroacupuncture decreases excessive alcohol consumption involving reduction of fosbδfosb levels in reward-related brain regions电针刺激减少过度饮酒涉及减少fosbδfosb水平与有偿性相关的大脑区域.pdf12页 内容提供方:xyz118 大小:1.36 MB 字数:约8.3万字 ...
Brains were processed and cut into 40μM thick, free-floating sections, which were then probed with anti-BrdU primary antibody (Sigma-Aldrich SAB4700630), followed by a biotin-conjugated secondary antibody (Vector Laboratories BA-2000). Sections were developed using diaminobenzidine and counterstained...