Results showed that the intensity of GFP fluorescence per cell or protoplast in diploid was generally brighter than in allotetraploid. Moreover, same hybridization signal was detected on allotetraploid and diploid plants by Southern blot analysis. By real-time RT-PCR and Western blot analysis, GFP ...
Understanding the properties of hybridisation lets us dive into the realms of science in a way that is hard to grasp in one go but excellent to study once we get to know more about it. Let us get to know more about the process of hybridization, which will help us understand the properti...
Conventional methods to characterize transgene integrations (e.g. fluores- cence in situ hybridization [FISH] or Southern blotting) lack the resolution to determine the integration site, and sequence-level integrity of both the transgene and host genome. We have shown previously that customizations ...
and hybridization. However, while agarose gel is typically used for electrophoresis in Southern blotting, formaldehyde or polyacrylamide gels are commonly used in Northern blotting. Southern blotting also requires DNA to be digested with restrictionenzymesbefore running the gel; this is not necessary ...
Recommended Lessons and Courses for You Related Lessons Related Courses Stability of Alkenes | Factors, Products & Examples Acid Catalyzed Hydration Concept & Examples Orbital Hybridization: Double & Triple Bond | Rules & Formula Butene Overview, Formula & Structure ...
Body of the Example Informative Speech Hybridization has been occurring in nature for thousands of years. Diaries of early hunters in the northwestern territories tell of shooting bears that were large and off-white with hairy paws, suggesting hybrids of Kodiak and polar bears. Recent DNA studies ...
Interspecific differentiation and intraspecific substructure in two closely related clupeids with extensive hybridization, Alosa alosa and Alosa fallax. J Fish Biol. 2006;69(sb):242–59. Article CAS Google Scholar Holzer AS, Sommerville C, Wootten R. Molecular studies on the seasonal occurrence ...
of the host cell involved in uptake of viruses [51–53]. This method involves cell transfection with short ‘interfering RNAs’ with complementarity to sequences of a target mRNA to be silenced. Their hybridization results in their degradation and consequently in downregulation of the encoded ...
Conventional methods to characterize transgene integrations (e.g. fluores- cence in situ hybridization [FISH] or Southern blotting) lack the resolution to determine the integration site, and sequence-level integrity of both the transgene and host genome. We have shown previously that customizations ...
Primers designed for unique and repetitive sequences allowed to reliably identify the sex of both larval and adult stages of the parasite. Conclusion Our genome-to-genome comparison method that we call "whole-genome in-silico subtractive hybridization" (WISH) allows for rapid identification of ...