Lecart, A. Deniset, P. Pernot, P. Eschwege, S. Ferlicot, S. Leveque-Fort, R. Briandet, and M. P. Fontaine- Aupart, "Ex vivo fluorescence imaging of normal and malig- nant urothelial cells to enhance early diagnosis," Photochem. Photobiol. 83, 1157-1166 (2007)....
such as Computed Tomography Angiography (CT-A) and duplex ultrasound (US) as part of the standard of care24. Relatively new in the (clinical) cardiovascular field is near-infrared fluorescence (NIRF) imaging. This modality uses fluorophores as imaging compound instead of the generally...
Fluorescence imaging of ex vivo murine carotids arteries.Raiyan T. ZamanHisanori KosugeGuillem PratxColin CarpenterLei XingMichael V. McConnell
In vivo Fluorescence Spectroscopy of Nonmelanoma Skin Cancer In vivo and ex vivo tissue autofluorescence (endogenous fluorescence) have been employed to investigate the presence of markers that could be used to detect tissue abnormalities and/or malignancies. We present a study of the autofluoresc......
n, A statistical comparison of AF647 fluorescence intensity in DMSO-, no hCG- and 4-MU-treated follicles at 9 h. P values: control versus no hCG = 1.24 × 10−4, control versus 4-MU = 1.15 × 10−5. In m,n, total n = 54; individual groups: DMSO n...
Here, the authors used a novel ex vivo retinal imaging preparation to examine the behavioral phenotype of living retinal microglia in intact tissue and in response to injury. METHODS: Fluorescence-labeled microglia in retinal explants from CX3CR1(+/GFP) transgenic mice were observed using time-...
Additionally, fur and skin pigmentation may severely compromise imaging due to tissue fluorescence absorption. To overcome these drawbacks of FMT imaging, we have developed an in- and ex vivo hybrid method for measuring GFR using the in vivo imaging system (IVIS). Methods:An IVIS-based imaging ...
In order to examine the bone microenvironment ex-vivo following the formation of breast cancer metastases, several techniques may be employed: fluorescence stereomicroscopy, magnetic resonance imaging (MRI), microCT (μCT), immunohistochemistry, and cytokine arrays, to name a few. These methods allow...
Fluorescence imaging of adult tissue cryosections Mice were anesthetized by isoflurane, and transcardially perfused with ice-cold phosphate-buffered saline followed by 4% paraformaldehyde (PFA). Organs were dissected and post-fixed in 4% PFA overnight. Organs were then cryopreserved in 30% sucrose ...
(Nile Red staining kit, Abcam, Cambridge, UK) as per manufacturer’s instructions. 8 fields for each condition were randomly acquired with an inverted fluorescence microscope (10x magnification; EVOS imaging system, Thermo-Fisher Scientific, Waltham, MA, US) and average fluorescence intensity per ...