This result unambiguously demonstrates the ability of the perfused brain tissue slices to utilize pyruvate within seconds of its arrival. The enzyme activities demonstrated by the appearance of [1-13C]lactate and [13C]bicarbonate are those of lactate dehydrogenase and pyruvate dehydrogenase, respectively...
2013. Enzyme- linked immunospot assays for direct ex vivo measurement of vaccine- induced human humoral immune responses in blood. Nat. Protoc. 8:1073-1087. http://dx.doi.org/10.1038/nprot.2013.058.Saletti G, Cuburu N, Yang JS, Dey A, Czerkinsky C. Enzyme-linked immunospot assays for ...
Furthermore, efficacy of interventions in (pre)clinical studies are almost exclusively based on the measurement of myocardial necrosis by assessing infarct size or necroenzyme release, which primarily represents the decay of cardiac myocytes, and which are major determinants of the outcome of AMI ...
Recombinant antibodies produced were purified from cell supernatant by Fast Protein Liquid Chromatography (FPLC) using a protein A column, and their concentration determined by absorbance measurement at 280 nm. Elisa 96-well ELISA plates (Maxisorp, Nunc) were coated with HLA-A2 monomers (overnight...
Ex-vivo antioxidant, enzyme inhibitory properties and computational analysis unveil the molecular mechanism of cardiac and penile phosphodiesterase-5 inhibition by bacterial strain HOKA1 extract as an aphrodisiac's agent 来自 dx.doi.org 喜欢 0 阅读量: 1 ...
This study aimed to develop a model of enzyme-induced IDD that could be used for efficient investigation of therapeutic treatment options in order to better mimic the clinical outcome of IDD humans. Such models could potentially allow for the replacement of in vivo animal models. Therefore, we ...
Ex vivo processing for maturation of Arabidopsis KDEL-tailed cysteine endopeptidase 2 (AtCEP2) pro-enzyme and its storage in endoplasmic reticulum derived ... (2014) Ex vivo processing for maturation of Arabidopsis KDEL-tailed cysteine endopeptidase 2 (AtCEP2) pro-enzyme and its storage in ...
Based on this research, we were originally intended to conduct ex vivo corneal and ex vivo scleral penetration experiments, while the activity of the cornea may limit the duration of penetration. Previous studies have demonstrated the existence of the tight junction protein claudin-1 in choroidal ...
The aim of this work was to determine if Hypodermin C (HC), an enzyme secreted by Hypoderma spp. larvae during their migration, is able to depress the lymphocyte response to mitogens and antigens. HC, when added to the lymphocyte culture, was able to influence the response of naive-calf...
We previously reported that normothermic ex vivo kidney perfusion (NEVKP) is superior in terms of organ protection compared to static cold storage (SCS), which is still the standard method of organ preservation, but the mechanisms are incompletely