2001 Mouse melanoma B16BL6 or human prostate cancer TSU cells were applied to the CAM; 10-day-old eggs metastatin colloidal solution 2 d after grafting, single dose [114] Zhang et al. 2010 HEp3-GFP tumour cells were applied directly to a filter-disc abraded area of the CAM (EDD 9)...
Membranes were then washed three times in TBST and incubated for at least 1 h with appropriate horseradish-peroxidase-conjugated secondary antibodies (Rabbit anti-sheep IgG (HL) HRP, cat#618620; Goat anti-mouse IgG (HL) HRP, cat#G21040; Goat anti-rabbit IgG (HL) HRP, cat#G21234; all...
mice were daily intraperitoneally treated with vinylcyclohexene dioxide (VCD, Sigma-Aldrich, St. Louis, MO, USA) for 19 days (100 μl/mouse at 160 mg/kg of VCD diluted in sesame oil) to induce ovarian failure34. Mice in the control group (n = 5) were treated with the vehicle...
Temporal expression of miRNAs and mRNAs in a mouse model of myocardial infarction Physiol. Genomics, 43 (2011), pp. 1087-1095 CrossrefView in ScopusGoogle Scholar 60 M. Tran, D. Tam, A. Bardia, M. Bhasin, G.C. Rowe, A. Kher, Z.K. Zsengeller, M.R. Akhavan-Sharif, E.V. Khan...
(mouse IgG1κ monoclonal, Dako, clone QBEnd/10). Slides were incubated with anti-CD68 (mouse IgG3κ monoclonal, Dako, clone PG-M1) for the staining of macrophages. Smooth muscle actin in the vessel walls was stained with anti-SMA (mouse monoclonal, Dako, clone 1A4). All slides were ...
Twenty-four hours later, cells were incubated with 4 μg/mL of anti-human CI-MPR mouse monoclonal antibody (Novusbio, cat. no. NB300-514) for 30 min in complete medium then fixed for 5 min with methanol at −20°C. Cells were then permeabilised/blocked for 1 h with blocking ...
1 µg/ml (P < 0.05) (Supplementary Fig.S2A). Meanwhile, BCG vaccination did not enhance the control of mycobacterial growth at any RIF concentrations (Supplementary Fig.S2B). Similar titration curves were observed with INH and RIF during co-culture with mouse splenocytes compared ...
In this study, we describe the use of rMVA-GFP, allowing detection of single rMVA-GFP infected cells with high sensitivity, to elucidate the tropism of the viral vector MVA.In vitrotropism was investigated by inoculating human PBMC with rMVA-GFP,ex vivotropism by inoculation of mouse lung expl...
In brief, for mouse MLR in Figure 5H, BALB/c splenocyte CD90.2+ T cells were plated in 96-well plate (flat-bottom) precoated with 1 μg/ml anti-CD3, followed by coculture with 8 days precultured irradiated C57BL/6 HSCs. For human MLR in Figure 7F, peripheral blood CD3+ cells ...
B Leon, G Martinez del Hoyo, V Parrillas, H Hernandez Vargas, P Sanchez-Mateos, N Longo, M Lopez-Bravo, C Ardavin Dendritic cell differentiation potential of mouse monocytes: monocytes represent immediate precursors of CD8− and CD8+ splenic dendritic cells Blood, 103 (2004), pp. 2001-228...