A cytogenetic study carried out by a direct method on the tumor of a patient with Ewing's sarcoma showed a t(11;22) in the six cells studied. The fact that this structural alteration was detected by a direct method indicates that the translocation is present in Ewing's sarcoma cells and...
was performed in 12 cases of Ewing sarcoma on the cDNA and/or genomic DNA breakpoint regions of a t(11;22)(q24;q12), which joins the EWS gene located on chromosome 22 with the FLI1 gene located on chromosome 11, in order to understand the molecular mechanism of this translocation. Rev...
一般认为,尤文氏肉瘤(Ewing's Sarcoma)病人大多是白人(高加索人),极少见于亚裔与非裔。由于病例少,业界不关注,至今没有靶向疗法或是免疫疗法。 人生就像个实验,有无数种假设等着人们去实践。那么些假设就是理想,而各种尝试与努力就是一个又一个的实验条件。有些人幸运,试那么一两次,就找到了方向,只要往那个...
The translocation, t(11;22)(q24;q12), recurrently observed in Ewing's sarcoma and in peripheral neuroepithelioma has been recently cloned. The analysis of a series of ES/PNE has revealed that the chromosome 22 breakpoints are clustered in a small region of 7 kb, called EWS...
Downing JR, et al. Detection of the (11;22)(q24;q12) translocation of Ewing’s sarcoma and peripheral neuroectodermal tumor by reverse transcription polymerase chain reaction. Am J Pathol. 1993;143(5):1294–300. CASPubMedPubMed CentralGoogle Scholar ...
Ewing sarcoma is an aggressive paediatric cancer of the bone and soft tissue. It results from a chromosomal translocation, predominantly t(11;22)(q24:q12), that fuses the N-terminal transactivation domain of the constitutively expressed EWSR1 protein with the C-terminal DNA binding domain of ...
In the most difficult cases, the differential diagnosis is based on cytogenetic studies to identify the genetic abnormalities typical of Ewing's sarcoma, notably (11;22) translocation and/or EWS/FLI1 or EWS/ERG fusion genes [1], [11], [12], [13]. These genetic abnormalities are not ...
Ewing Sarcoma 11;22 Translocation Produces a Chimeric Transcription Factor that Requires the DNA-Binding Domain Encoded by FLI1 for ... Changes in incidence and survival of Ewing sarcoma patients over the past 3 decades: Surveillance Epidemiology and End Results data. ...
The most frequent translocation—t(11;22)(q14;q12), 85% of cases—results in the expression of the EWS-FLI1 chimeric protein [2]. Ewing sarcoma is a very aggressive tumor, and its treatment requires either surgery and/or radiation therapy for local control, along with intensive chemotherapy...
However, the cell of origin of Ewing's sarcoma is unknown. Most cases of Ewing's sarcoma have a recurrent chromosomal translocation, t(11;22)(q24;q12), that encodes a fusion protein, EWS/FLI (Dellatre et al., 1992). The FLI portion contains an ETS family DNA-binding domain while ...