Crosslinking was terminated by the addition of 1/10 volume 1.25 M glycine for 5 min at room temperature followed by cell lysis (1% SDS, 10 mM EDTA, 50 mM Tris, pH 8) for 10 min and sonication (Misonix, Microson Ultrasonic Cell Disruptor (20 s on, 40 off, 10 amplitude for 30 min...
Schematic diagram of the experimental timeline. The Lacto or Placebo supplementation started when birds were 1 day old and lasted 9 weeks. The stress treatment spanned weeks 11–13. Physical examinations and measurements of behaviour, blood samples, and cecal droppings samples were conducted at 10,...
Cells were also fixed in 4% paraformaldehyde, 15 min- utes, room temperature, and indirect immunofluorescence analysis to detect CCN2 was performed using an anti-CCN2 antibody (Santa Cruz Biotechnology) and a Texas Red-conju- gated secondary antibody (Jackson Immunoresearch, West Grove, PA, USA...
Samples dedicated to serve as a positive control (Figure S2E) were incubated with DNase I diluted 1:100 in distilled water for 15 minutes at room temperature. For each sample, 5 μl TdT enzyme solution were mixed with 45 μl label solution of the In situ Cell Death Detection Kit. Guts...
Probes (5 × 104 cpm) were added and the incubation continued at room temperature for 20 min. Polyacrylamide gels (5%) containing 2.5% (v/v) glycerol and 0.5% Tris borate/EDTA were prerun in 0.5% Tris borate/EDTA buffer at 4 °C for 30 min at 300 volts. After loading the samples,...
a, Experimental schematic for studying the effects ofETS2overexpression. The diagram was created using BioRender.b,ETS2mRNA levels in transfected (n = 8) or untransfected (from a separate experiment) macrophages. Data are mean ± s.e.m. CPM, counts per million.c, fGSEA analysis ...
Cells were fixed in methanol for 20 min at −20 °C, washed 3 × with PBS and blocked for 1 h at room temperature in PBS with 0.3% Tween-20 and 5% BSA. The primary antibody was Ets-1 (Bethyl), which was diluted 1:100 in PBS with 0.3% Tween-20 and 1% BSA (antibody ...
Figure 8.Schematic diagram of BTBT generation with different gate inputs in MAJ logic device. (a)ABC= “011”, (b)ABC= “110”, (c)ABC= “101”, and (d)ABC= “111”. To build a static complementary MAJ logic gate, a P-type TFET with MAJ behavior is also indispensable. The desi...