After 48 h, media was removed, and cells were washed two times with  1 PBS, resuspended in 500 ml  1 PLB, disrupted by one freeze/thaw cycle ( À 80 °C) and dissociated with a BD 1 ml 26G syringe. Luciferase activity was measured in 20 ml of cell lysate using a BD ...
Heating source Heating method Infrared heating tube Max temperature Substrate temperatures up to 60ºC Max heating power 1000W Syringe pump Syringe capacity 2.5ml~60ml Flow range 2.779μl/min~72.24ml/min Collector Mandrel collector ...
19.A method of treatment of a chronic inflammatory disease in a subject, the method comprising administering to the subject an effective amount of an agent which beneficially alters the activity or expression of ets-2. 20.The method of claim 19, wherein the agent is selected from the group ...
[PMSF]) and allowed to swell on ice for 15 minutes. Swollen cells were then passed through a 25-gauge needle attached to a 1-mL syringe (prerinsed with buffer A). The cells were drawn slowly into the syringe from the microfuge tube and then ejected with a single stroke. Five strokes ...
The suspensions were magnetically stirred (1400 rpm) and aliquots were collected at increasing times, using a 10×10−610×10−6 m3 syringe coupled to a Teflon tube, filtered through a Millipore membrane with pore size of 0.45×10−60.45×10−6 m (previously washed with nitric acid ...