Sixty microliters ofhydroxylaminereagent (500mMhydroxylamine hydrochloride, 25mMEDTA, titrated to pH 7.5 by stepwise addition of solid Na2CO3) is added to the antibody–SATP stock in buffer B (100μl, 0.15mg/ml) to deprotect the SH-group, vortexed for 2min, and incubated for 1h. Cantilever...