To measure the absorption spectrum of a dye-protein conjugate, it is recommended to keep the sample concentration in the range of 1-10 µM depending on the extinction coefficient of the dye. Read OD (absorbance) at 280 nm and dye maximum absorption (ƛmax = 853 nm for iFluor™ 860...
The presence of O-acetyl ester on fucogalactan was suggested by IR spectrum and confirmed by gas-liquid chromatographic analysis. The determination of acetyl ester revealed the complete molar composition of fucogalactan to be fucose: galactose: acetic acid = 1: 1:0.6. Sequential Smith degradation ...
13.There are two reactive groups, amino and butoxy in the borate ester molecule.The structure of the borate ester was characterized by IR spectrum. [中文文摘]通过分子结构设计,合成了种氮-硼内配位结构硼酸偶联剂,解决了硼酸水解稳定性差问题,同时分子结构中含有氨基、丁基等活性基团。 14.Archaean cell...
ChemicalBook Provide 3-Oxobutyric acid menthyl ester(1144-50-9) IR,IR2,MS,IR3,IR,1H NMR,Raman,ESR,13C NMR,Spectrum
ChemicalBook Provide 2-Bromobutyric acid methyl ester(3196-15-4) IR,IR2,MS,IR3,IR,1H NMR,Raman,ESR,13C NMR,Spectrum
Cyanines are used in a wide variety of biological applications including comparative genomic hybridization and in gene chips. Depending on the structure, they cover the spectrum visible and IR wavelengths. Cy 3, Cy5 and Cy7 are the most popular cyanine dyes, in particular Cy3 and Cy5 are typic...
AI Tutor Topic summary Created using AI Acetoaceticestersundergo various reactions starting with deprotonation to form an enolate. This enolate can react with alkyl halides for alkylation, allowing for dialkylation through sequential reactions. Cycloalkylation occurs with terminal a...
The IR indicated hydroxy groups (3614 cm−1) and two carbonyls (1715, 1701 cm−1). The 1H NMR spectrum (Table S2, SI) contained two very similar sets of signals, in ca. 1:1 ratio, suggesting diastereoisomers which interconverted slowly on the 1H NMR timescale. This may indicate a...
Alexa Fluor™ 700 is a bright and photostable near-IR dye that can be excited with a xenon-arc lamp, far-red diode lasers, or dye pumped lasers operating in the 675–700 nm range. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 700 dye is water ...
(i) IR spectrum (KBr, cm-1): 1740, 1635, 1520, 1400, 1340, 1300, 1200, 1090. (ii) Specific rotation [α]D26: -25,2 (c=1.0, MeOH). (iii) NMR spectrum (DMSO-d6, δ): 0.60-1.04 (3H, m); 1.04-1.76 (8H, m); 1.76-2.16 (2H, m); 2.16-2.48 (2H, m); 2.60-3.04 ...