Each reaction was performed in trip- licate, with a control reaction using equivalent starting volume of RNA to verify absence of contaminating DNA. Primers to amplify an ∼50–150 bp region within each transcript were designed using Primer Express 3.0 soft- ware, and qRT-PCR was ...
Each reaction was performed in trip- licate, with a control reaction using equivalent starting volume of RNA to verify absence of contaminating DNA. Primers to amplify an ∼50–150 bp region within each transcript were designed using Primer Express 3.0 soft- ware, and qRT-PCR was ...