Under a Creative Commons license open accessHighlights Summary Because the esophageal epithelium lacks a defined stem cell niche, it is unclear whether all basal epithelial cells in the adult esophagus are functionally equivalent. In this study, we showed that basal cells in the mouse esophagus conta...
Epithelium of the ventral side was imaged by a confocal LSM710 confocal laser scanning microscope with ×25/0.8 NA Imm objectives (Carl Zeiss). Images were acquired under the following conditions: 1024 × 1024 pixel size in x, y 0.332 µm, 2.000 µm z-section. Images were...
Six L2-IL1B mice with advanced stage of disease (12–16 months old) were injected with the CXCR4-targeted, Sulfo-Cy5-labeled peptide (MK007), and ex vivo wide-field imaging of the whole stomach was performed 4 h after injection. Before ex vivo imaging, fluorescence endoscopy was performed...
sections were preincubated with normal nonimmune serum and then tested by avidin–biotin complex (ABC) using a standard ABC immunoperoxidase method. Briefly, after washing in Tris-phosphate buffered saline (TPBS), pH 7.6, the sections were incubated with a biotin-labeled secondary antibody, followe...