Bioinformatic analysisThe sea dragon Solenognathus hardwickii has long been used as a traditional Chinese medicine for the treatment of various diseases, such as male impotency. To gain a comprehensive insight into the protein components of the sea dragon, shotgun proteomic analysis of its protein ...
摘要: The protein expression profiling of sea dragon,Solenognathus hardwickii,was examined using shotgun proteomic analysis. More than 800 proteins were identified and ribosomal protein synthesis was found to play an important role in the possible signal network of the identified proteins.关键词: ...
(10) was amplified from genomic DNA, cloned into pET15b, and the recombinant protein purified from a bacterial expression system. Electrospray mass spectrometry of both the recombinant material and the native form of CMO isolated from Acinetobacter yielded molecular weights within 0.01% of those ...
(Fig.1c). The one-step, clean-up free protocol allowed the direct injection of the labelledN-glycans into the CE-ESI-MS. The applicability of hydrazide labels has been shown before in the analysis of releasedN-glycans by LC-ESI-MS and MALDI-TOF-MS methods, enhancing ionization and ...
Bottom-up proteomics is a mass spectrometric (MS)-based approach for the characterization of peptides obtained from in-solution protein digestion. MS is favored over other methods for peptide and protein analysis because of its better sensitivity and high throughput. Inorganic ions and surfactants pre...
Matrix-assisted laser desorption/ionization mass spectroscopy (MALDI-MS) is a rapid and sensitive analytical tool well suited for obtaining molecular weights of peptides and proteins from complex samples. Here, a fast and simple approach to cellular protein profiling is described in which mammalian ...
ESI-MS for peptides应用说明说明书 Applications of ESI-MS for peptides •All synthetically prepared peptides should be analyzed by ESI-MS.•Guarantees of purity based on observation of “a single peak by reversed-phase HPLC” and by “it gave the correct sequence when analyzed by Edman ...
Acetonitrile was used for the LC-MS analysis because of its stronger eluting power in comparison to methanol. This decreased the washing time of the LC column. The rest of the analysis parameters are the same for the FIA and LC analysis. Matrix effect evaluation Phospholipids are a major ...
Proteomics is a vast new field that is defined by global or high-throughput analysis of many proteins to gain a better understanding of cell function and dysfunction. Currently, we are developing a microfluidic system for two-dimensional separations of intact proteins with on-line ESI-MS analysis...
of analysis were less than 6.7%, and the accuracy (R.E.) was within ±9.0% at the concentrations of 5, 20, 200, and 2000ng/mL. The total run time was 5.0min. This method was successfully applied for clinical pharmacokinetic investigation. 展开 关键词: Docetaxel LC/ESI–MS/MS Rapid ...