The LC-MS Workflow: Is MALDI or ESI the Preferable Ionization Method?Anders TangenHanno EhringJohn FlensburgHenrik Wadensten
摘要:目的建立HPLC-ESI-MS/MS法同时测定连翘心中连翘酯苷A、连翘苷、连翘脂素、芦丁、槲皮素、异槲皮苷、阿魏酸和橙皮苷的量。方法色谱柱为Diamonsil C18柱(150 mm×4.6 mm,5 μm),流动相为甲醇-0.1%甲酸水,梯度洗脱,体积流量800 μL/min,进样量10 μL;质谱采用电喷雾离子源(ESI源),负离子模式监测,源...
In a context where the commercial and nutritional interest in insect chitin is always increasing, an accurate and precise method to quantify this biopolyme
ESI is the most commonly used ionization method for the LC-MS/MS technique, and does not always require a derivatization step. However, because neutral sterols are poorly ionized by electrospray, the charged moieties were introduced into the 3β-hydroxyl group of the sterols as an N-methylpyrid...
A valid method using liquid chromatography coupled with electrospray ionization (ESI) and ion trap mass spectrometry was established for the study of the a... P Jiang,R Liu,S Dou,... - 《Biomedical Chromatography》 被引量: 277发表: 2010年 ...
A sensitive, simple, rapid, and specific liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) method was developed and validated for the identification and quantification of losartan in a small volume of human plasma. Losartan and I.S. were successfully separated on a CN ...
(MeCN) B: 5 mM TDFHA in water Flow: 0.2 ml/min Injection: 500 μl Ionization: Electrospray positive ion mode Fragmentor: 80 V Nebulizer: 60 psig nitrogen Drying gas: 10 l/min nitrogen at 350 °C The developed method was an efficient and reliable means for the quantitation of three ...
The workflows use optimized Orbitrap IRMS data-extraction and -processing software and are transferable to various analytes amenable to soft ionization, including metabolites, peptides, drugs and environmental pollutants. Optimized mass spectrometry systems will enable intramolecular isotope research in many ...
The method is based on the separation of the cardenolides using high performance liquid chromatography (HPLC) followed by detection with electrospray ionization mass-spectrometry (ESI-MS). Chromatographic separation of the analytes was achieved on a RP C-18 column using water: 1% formic acid in ...
目的 通过建立UPLC-ESI-IT-TOF/MS分析方法对芪苓温肾消囊方提取物化学成分进行分析,结合文献报道,初步推测可能的起效成分。方法 采用Waters ACQUITY UPLC BEH C18色谱柱(100 mm×2.1 mm,1.7μm);以乙腈-0.1%甲酸水溶液为流动相梯度洗脱;体积流量为0.4 mL/min;ESI