The roles of the ESCRT pathway in eukaryotic cells include regulation of cargo-containing vesicles bud into endosomes to form multivesicular bodies (MVBs)2, cytokinesis3, exosome secretion4, and autophagy5. The ESCRT components (also known as class E proteins) comprise five distinct com- plexes, ...
Here we identify that RAB31 marks and controls an ESCRT-independent exosome pathway. Active RAB31, phosphorylated by epidermal growth factor receptor (EGFR), engages flotillin proteins in lipid raft microdomains to drive EGFR entry into MVEs to form ILVs, which is independent of the ESCRT (...
Exosomes are generated within the multivesicular endosomes (MVEs) as intraluminal vesicles (ILVs) and secreted during the fusion of MVEs with the cell membrane. The mechanisms of exosome biogenesis remain poorly explored. Here we identify that RAB31 mark
5. Henne W M, Buchkovich N J, Emr S D. The ESCRT pathway. Dev Cell, 2011, 21(1): 77-91 6. Trajkovic K, Hsu C, Chiantia S, et al. Ceramide triggers budding of exosome vesicles into multivesicular endosomes. Science, 2008, 319(5867): 1244-1247 7. Kowal J, Tkach M, Thery C...
Here we identify that RAB31 marks and controls an ESCRT-independent exosome pathway. Active RAB31, phosphorylated by epidermal growth factor receptor (EGFR), engages flotillin proteins in lipid raft microdomains to drive EGFR entry into MVEs to form ILVs, which is independent of the ESCRT (...
Get on the exosome bus with ALIX. Nat Cell Biol, 2012, 14(7):654-655 [58] Babst M, Sato T K, Banta L M, et al. Endosomal transport function in yeast requires a novel AAA-type ATPase, Vps4. EMBO J, 1997, 16(8):1820-1831 [59] Babst M, Wendland B, Estepa E J, et al...
The cellular endosomal sorting complex required for transport (ESCRT) pathway is a multifunctional pathway involved in cell physiological activities. While the majority of RNA viruses bearing L-domains are known to hijack the ESCRT pathway to complete th
Exosome for mRNA delivery: strategies and therapeutic applications 2024, Journal of Nanobiotechnology An Overview of Nanoparticle-Based Delivery Platforms for mRNA Vaccines for Treating Cancer 2024, Vaccines Lipid Nanoparticle (LNP) Delivery Carrier-Assisted Targeted Controlled Release mRNA Vaccines in Tumor ...
11. Marina Vietri, et al. The many functions of ESCRTs. Nat Rev Mol Cell Biol. 2020 Jan;21(1):25-42. 12.Matias Ostrowski, et al. Rab27a and Rab27b control different steps of the exosome secretion pathway. Nat Cell Biol. 2010 Jan;12(1):19-30; sup pp 1-13....
Sorting of integral membrane protein cargoes to the intraluminal vesicles (ILVs) of multivesicular endosomes (MVEs) is a key step in many physiological processes including cessation of growth factor signaling, lysosomal degradation, exosome secretion, lysosome related organelle (LRO) biogenesis, and perh...